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Multi-parameter quantitative mapping of microfluidic devices

หน่วยงาน Edinburgh Research Archive, United Kingdom

รายละเอียด

ชื่อเรื่อง : Multi-parameter quantitative mapping of microfluidic devices
นักวิจัย : Bennet, Mathieu A.
คำค้น : microfluidics , fluorescence , lifetime imaging microscopy , optical trap , particle imaging velocimetry , multimodality imaging
หน่วยงาน : Edinburgh Research Archive, United Kingdom
ผู้ร่วมงาน : Jones, Anita , Dryden, David , Engineering and Physical Sciences Research Council (EPSRC)
ปีพิมพ์ : 2554
อ้างอิง : http://hdl.handle.net/1842/5739
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : EMPC2011, Oral Presentation, Brighton (England) Sep 2011 Characterisation of ion transportation during electroplating of high aspect ratio microvias using megasonic agitation S.Costello, N.Strusevich, M.K.Patel, C.Bailey, D.Flynn, R.W. Kay, D. Price, M.Bennet, A.C.Jones, R. Habeshaw, C. Demore, S. Cochran and M.P.Y. Desmulliez , Mathieu A. Bennet, Patricia R. Richardson, Jochen Arlt, Aongus McCarthy, Gerald S. Buller and Anita C. Jones . Optically trapped microsensors for microfluidic temperature measurement by fluorescence lifetime imaging microscopy, Lab on a Chip, August 2011, DOI: 10.1039/c1lc20391f.
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to non-invasively map the physical and chemical environment within microfluidic devices. In this work FLIM has been used in conjunction with a variety of other techniques to provide a greater insight into flow behaviour and fluid properties at the microscale. The pH-sensitive fluorescent dyes, fluorescein and C-SNARF 1, have been used to generate pH maps of microfluidic devices with a time-gated camera and a time-and-space-correlated single photon counting (TSCSPC) detector, respectively. Using time-gated detection and fluorescein, the fluorescence lifetime images allow for direct reading of the pH. The relative contribution to fluorescence of the acid and basic forms of C-SNARF 1 was spatially resolved on the basis of pre-exponential factors, giving quantitative mapping of the pH in the microfluidic device. Three dimensional maps of solvent composition have been generated using 2-photon excitation FLIM (2PE-FLIM) in order to observe the importance of gravitational effects in microfluidic devices. Two fluidic systems have been studied: glycerol concentration in the microfluidic device was measured using Kiton red; water concentration in a methanolic solution was measured using ANS. The density mismatch between two solutions of different composition induced a rotation of the interface between two streams travelling side by side in a microchannel. The experiment has provided evidence of non-negligible gravitational effects in microflows. 2PE-FLIM has superior capability than methods used previously to assess similar phenomena. FLIM and micro-particle imaging velocimetry (μ-PIV) have been implemented on a custom-built open frame microscope and used simultaneously for multimodal mapping of fluid properties and flow characteristics. It has been shown that viscosity mismatch between two streams induces a non-constant advective transport across the channel and results in a flow profile that deviates from the usual Poiseuille profile, characteristic of pressure driven flow in microfluidic devices.

บรรณานุกรม :
Bennet, Mathieu A. . (2554). Multi-parameter quantitative mapping of microfluidic devices.
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom .
Bennet, Mathieu A. . 2554. "Multi-parameter quantitative mapping of microfluidic devices".
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom .
Bennet, Mathieu A. . "Multi-parameter quantitative mapping of microfluidic devices."
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom , 2554. Print.
Bennet, Mathieu A. . Multi-parameter quantitative mapping of microfluidic devices. กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom ; 2554.