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Regulation of microtubule nucleation in Schizosaccharomyces pombe: recruitment of Mto1 to the site of the prospective eMTOC

หน่วยงาน Edinburgh Research Archive, United Kingdom

รายละเอียด

ชื่อเรื่อง : Regulation of microtubule nucleation in Schizosaccharomyces pombe: recruitment of Mto1 to the site of the prospective eMTOC
นักวิจัย : Miller, Victoria Jane
คำค้น : fission yeast , γ-tubulin complex , centrosomin-related proteins , microtubule array
หน่วยงาน : Edinburgh Research Archive, United Kingdom
ผู้ร่วมงาน : Sawin, Ken , Hardwick, Kevin , Biotechnology and Biological Sciences Research Council (BBSRC)
ปีพิมพ์ : 2553
อ้างอิง : http://hdl.handle.net/1842/4904
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Samejima, I., Miller, V.J., Groocock, L.M., and Sawin, K.E. (2008). Two distinct regions of Mto1 are required for normal microtubule nucleation and efficient association with the gamma-tubulin complex in vivo. J Cell Sci 121, 3971-3980.
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Mto1 recruits γ-tubulin to the sites of cytoplasmic microtubule nucleation in the fission yeast Schizosaccharomyces pombe. The regulation of Mto1 localisation is central to re-modelling of the microtubule cytoskeleton during the cell cycle. This thesis describes how Mto1 is recruited to the cell equator during mitosis, thereby establishing the equatorial microtubule nucleation centre (eMTOC). F-actin is found to be required for Mto1 localisation to the cell equator and Mto1 is shown to co-localise with the cytokinetic actin ring (CAR). Yeast 2-hybrid screening and tandem-affinity purification were used to screen for additional proteins required for Mto1 localisation to the equator. Further candidate screening identified Myp2, a type II myosin present in the CAR, as being required for Mto1 localisation to the cell equator. Myp2 is shown to physically interact with Mto1 and to be required for formation of the post-anaphase microtubule array. The regulation of Mto1 localisation to the cell equator was also studied. Time-lapse microscopy reveals that Mto1 localisation to the equator does not require either the anaphase-promoting complex or the septation initiation network, both of which have been previously shown to be necessary for the recruitment of γ-tubulin to the eMTOC. Maintenance of the equatorial CAR has previously been attributed to the postanaphase array. The position of the CAR in the mto1-427 mutant strain, which fails to nucleate a PAA, is shown to be unaltered from wild-type strain during exponential growth, suggesting that the PAA does not centre the CAR during normal growth.

บรรณานุกรม :
Miller, Victoria Jane . (2553). Regulation of microtubule nucleation in Schizosaccharomyces pombe: recruitment of Mto1 to the site of the prospective eMTOC.
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom .
Miller, Victoria Jane . 2553. "Regulation of microtubule nucleation in Schizosaccharomyces pombe: recruitment of Mto1 to the site of the prospective eMTOC".
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom .
Miller, Victoria Jane . "Regulation of microtubule nucleation in Schizosaccharomyces pombe: recruitment of Mto1 to the site of the prospective eMTOC."
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom , 2553. Print.
Miller, Victoria Jane . Regulation of microtubule nucleation in Schizosaccharomyces pombe: recruitment of Mto1 to the site of the prospective eMTOC. กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom ; 2553.