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Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells

หน่วยงาน Edinburgh Research Archive, United Kingdom

รายละเอียด

ชื่อเรื่อง : Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells
นักวิจัย : Niwa, Hitoshi , Masui, Shinji , Chambers, Ian , Smith, Austin G , Miyazaki, Jun-ichi
คำค้น : Phenotypic Complementation , Embryonic , Stem Cells , gene
หน่วยงาน : Edinburgh Research Archive, United Kingdom
ผู้ร่วมงาน : -
ปีพิมพ์ : 2544
อ้างอิง : MOLECULAR AND CELLULAR BIOLOGY, Mar. 2002, p. 1526–1536 , DOI: 10.1128/MCB.22.5.1526–1536.2002 , http://hdl.handle.net/1842/645
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : 11
บทคัดย่อ/คำอธิบาย :

Transcription factors of the POU family govern cell fate through combinatorial interactions with coactivators and corepressors. The POU factor Oct-3/4 can define differentiation, dedifferentation, or self-renewal of pluripotent embryonic stem (ES) cells in a sensitive, dose-dependent manner (H. Niwa, J.-I. Miyazali, and A. G. Smith, Nat. Genet. 24:372–376, 2000). Here we have developed a complementation assay based on the ability of Oct-3/4 transgenes to rescue self-renewal in conditionally null ES cells and used this to define which domains of Oct-3/4 are required to sustain the undifferentiated stem cell phenotype. Surprisingly, we found that molecules lacking either the N-terminal or C-terminal transactivation domain, though not both, can effectively replace full-length Oct-3/4. Furthermore, a fusion of the heterologous transactivation domain of Oct-2 to the Oct-3/4 POU domain can also sustain self-renewal. Thus, the unique function of Oct-3/4 in ES cell propagation resides in combination of the specific POU domain with a generic proline-rich transactivation domain. Interestingly, however, Oct-3/4 target gene expression elicited by the N- and C-terminal transactivation domains is not identical, indicating that at least one class of genes activated by Oct-3/4 is not required for ES cell propagation.

บรรณานุกรม :
Niwa, Hitoshi , Masui, Shinji , Chambers, Ian , Smith, Austin G , Miyazaki, Jun-ichi . (2544). Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells.
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom .
Niwa, Hitoshi , Masui, Shinji , Chambers, Ian , Smith, Austin G , Miyazaki, Jun-ichi . 2544. "Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells".
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom .
Niwa, Hitoshi , Masui, Shinji , Chambers, Ian , Smith, Austin G , Miyazaki, Jun-ichi . "Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells."
    กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom , 2544. Print.
Niwa, Hitoshi , Masui, Shinji , Chambers, Ian , Smith, Austin G , Miyazaki, Jun-ichi . Phenotypic Complementation Establishes Requirements for Specific POU Domain and Generic Transactivation Function of Oct-3/4 in Embryonic Stem Cells. กรุงเทพมหานคร : Edinburgh Research Archive, United Kingdom ; 2544.