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Identification and characterization of an eIF4e DNA aptamer that inhibits proliferation with high throughput sequencing

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Identification and characterization of an eIF4e DNA aptamer that inhibits proliferation with high throughput sequencing
นักวิจัย : Guo, Wei Mei , Kong, Kiat Whye , Brown, Christopher John , Quah, Soo Tng , Yeo, Hui Ling , Hoon, Shawn , Seow, Yiqi
คำค้น : DRNTU::Science::Biological sciences::Molecular biology
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Guo, W. M., Kong, K. W., Brown, C. J., Quah, S. T., Yeo, H. L., Hoon, S., et al. (2014). Identification and characterization of an eIF4e DNA aptamer that inhibits proliferation with high throughput sequencing. Molecular therapy-nucleic acids, 3. , 2162-2531 , http://hdl.handle.net/10220/25386 , http://dx.doi.org/10.1038/mtna.2014.70
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Molecular therapy-nucleic acids
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Development of DNA aptamer screens that are both simple and informative can increase the success rate of DNA aptamer selection and induce greater adoption. High eIF4e levels contribute to malignancies, thus eIF4e presents itself as a valuable target for DNA aptamer-based inhibition screen. Here, we demonstrate a method for the rapid selection of looped DNA aptamers against eIF4e by combining negative selection and purification in a single step, followed by characterization with high throughput sequencing. The resulting aptamers show functional binding to eIF4e and inhibit translation initiation in biochemical assays. When transfected into cells, eIF4e aptamers cause a dramatic loss of cell proliferation in tumor cells as seen with eIF4e knockdown with antisense oligonucleotides, shRNAs, and siRNAs, hinting at therapeutic possibilities. With the large data set provided by high throughput sequencing, we demonstrate that selection happens in waves and that sequencing data can be used to infer aptamer structure. Lastly, we show that ligation of looped aptamers can enhance their functional effects. These results demonstrate a rapid protocol to screen and optimize aptamers against macromolecules of interest.

บรรณานุกรม :
Guo, Wei Mei , Kong, Kiat Whye , Brown, Christopher John , Quah, Soo Tng , Yeo, Hui Ling , Hoon, Shawn , Seow, Yiqi . (2557). Identification and characterization of an eIF4e DNA aptamer that inhibits proliferation with high throughput sequencing.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Guo, Wei Mei , Kong, Kiat Whye , Brown, Christopher John , Quah, Soo Tng , Yeo, Hui Ling , Hoon, Shawn , Seow, Yiqi . 2557. "Identification and characterization of an eIF4e DNA aptamer that inhibits proliferation with high throughput sequencing".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Guo, Wei Mei , Kong, Kiat Whye , Brown, Christopher John , Quah, Soo Tng , Yeo, Hui Ling , Hoon, Shawn , Seow, Yiqi . "Identification and characterization of an eIF4e DNA aptamer that inhibits proliferation with high throughput sequencing."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Guo, Wei Mei , Kong, Kiat Whye , Brown, Christopher John , Quah, Soo Tng , Yeo, Hui Ling , Hoon, Shawn , Seow, Yiqi . Identification and characterization of an eIF4e DNA aptamer that inhibits proliferation with high throughput sequencing. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.