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Functional mapping of the zebrafish early embryo proteome and transcriptome

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Functional mapping of the zebrafish early embryo proteome and transcriptome
นักวิจัย : Alli Shaik, Asfa , Wee, Sheena , Li, Rachel Hai Xia , Li, Zhen , Carney, Tom J. , Mathavan, Sinnakaruppan , Gunaratne, Jayantha
คำค้น : DRNTU::Science::Biological sciences::Zoology::Vertebrates
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Alli Shaik, A., Wee, S., Li, R. H. X., Li, Z., Carney, T. J., Mathavan, S., et al.(2014). Functional mapping of the zebrafish early embryo proteome and transcriptome. Journal of proteome research, 13(12), 5536-5550. , http://hdl.handle.net/10220/25306 , http://dx.doi.org/10.1021/pr5005136
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of proteome research
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Zebrafish is a popular system for studying vertebrate development and disease that shows high genetic conservation with humans. Molecular level studies at different stages of development are essential for understanding the processes deployed during ontogeny. Here, we performed comparative analysis of the whole proteome and transcriptome of the early stage (24 h post-fertilization) zebrafish embryo. We identified 8363 proteins with their approximate cellular abundances (the largest number of zebrafish embryo proteins quantified thus far), through a combination of thorough deyolking and extensive fractionation procedures, before resolving the peptides by mass spectrometry. We performed deep sequencing of the transcripts and found that the expressed proteome and transcriptome displayed a moderate correlation for the majority of cellular processes. Integrative functional mapping of the quantified genes demonstrated that embryonic developmental systems differentially exploit transcriptional and post-transcriptional regulatory mechanisms to modulate protein abundance. Using network mapping of the low-abundance proteins, we identified various signal transduction pathways important in embryonic development and also revealed genes that may be regulated at the post-transcriptional level. Our data set represents a deep coverage of the functional proteome and transcriptome of the developing zebrafish, and our findings unveil molecular regulatory mechanisms that underlie embryonic development.

บรรณานุกรม :
Alli Shaik, Asfa , Wee, Sheena , Li, Rachel Hai Xia , Li, Zhen , Carney, Tom J. , Mathavan, Sinnakaruppan , Gunaratne, Jayantha . (2557). Functional mapping of the zebrafish early embryo proteome and transcriptome.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Alli Shaik, Asfa , Wee, Sheena , Li, Rachel Hai Xia , Li, Zhen , Carney, Tom J. , Mathavan, Sinnakaruppan , Gunaratne, Jayantha . 2557. "Functional mapping of the zebrafish early embryo proteome and transcriptome".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Alli Shaik, Asfa , Wee, Sheena , Li, Rachel Hai Xia , Li, Zhen , Carney, Tom J. , Mathavan, Sinnakaruppan , Gunaratne, Jayantha . "Functional mapping of the zebrafish early embryo proteome and transcriptome."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Alli Shaik, Asfa , Wee, Sheena , Li, Rachel Hai Xia , Li, Zhen , Carney, Tom J. , Mathavan, Sinnakaruppan , Gunaratne, Jayantha . Functional mapping of the zebrafish early embryo proteome and transcriptome. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.