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Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin

หน่วยงาน Central Queensland University, Australia

รายละเอียด

ชื่อเรื่อง : Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin
นักวิจัย : Liu, Ying. , Østrup, Olga. , Li, Juan. , Vajta, Gabor. , Lin, Lin. , Kragh, Peter M. , Purup, Stig. , Hyttel, Poul. , Callesen, Henrik.
คำค้น : 970111 Expanding Knowledge in the Medical and Health Sciences. , 060499 Genetics not elsewhere classified. , Permeabilization -- Porcine -- Pre-treatment -- Somatic cell nuclear transfer -- Xenopus egg extract , Journal Article. Refereed, Scholarly Journal
หน่วยงาน : Central Queensland University, Australia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : http://hdl.cqu.edu.au/10018/1019647
ที่มา : Liu, Y, Østrup, O, Li, J, Vajta, G, Lin, L, Kragh, PM, Purup, S, Hyttel, P & Callesen, H 2012, 'Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin', Zygote, vol. 20, no. 1, pp. 61-66, http://dx.doi.org/10.1017/S096719941000064X
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Zygote. United Kingdom : Cambridge University Press, 2012. Vol. 20, no. 1 (February 2012), p. 61-66 6 pages Refereed 0967-1994 1469-8730 (online) , ACQUIRE [electronic resource] : Central Queensland University Institutional Repository.
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Pre-treating donor cells before somatic cell nuclear transfer (SCNT, ‘cloning’) may improve the efficiency of the technology. The aim of this study was to evaluate the early development of cloned embryos produced with porcine fibroblasts pre-treated with a permeabilizing agent and extract from Xenopus laevis eggs. In Experiment 1, fetal fibroblasts were permeabilized by digitonin, incubated in egg extractand, after re-sealing of cell membranes, cultured for 3 or 5 days before use as donor cells in handmade cloning (HMC). Controls were produced by HMC with non-treated donor cells. The blastocyst rate for reconstructed embryos increased significantly when digitonin-permeabilized, extract-treated cells were used after 5 days of culture after re-sealing. In Experiment 2, fetal and adult fibroblasts were treated with digitonin alone before re-sealing the cell membranes, then cultured for 3 or 5 days and used as donor cells in HMC. Treatment with digitonin alone increased the blastocyst rate, but only when fetal, and not adult fibroblasts, were used as donor cells, and only after 3 days of culture. In conclusion, we find a time window for increased efficiency of porcine SCNT using donor cells after pre-treatment with permeabilization/re-sealing and Xenopus egg extract. Interestingly, we observe a similar increase in cloning efficiency by permeabilization/re-sealing of donor cells without extract treatment that seems to depend on choice of donor cell type. Thus, pre-treatment of donor cells using permeabilizing treatment followed by re-sealing and in vitro culture for few days could be a simple way to improve the efficiency of porcine cloning.

บรรณานุกรม :
Liu, Ying. , Østrup, Olga. , Li, Juan. , Vajta, Gabor. , Lin, Lin. , Kragh, Peter M. , Purup, Stig. , Hyttel, Poul. , Callesen, Henrik. . (2555). Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin.
    กรุงเทพมหานคร : Central Queensland University, Australia.
Liu, Ying. , Østrup, Olga. , Li, Juan. , Vajta, Gabor. , Lin, Lin. , Kragh, Peter M. , Purup, Stig. , Hyttel, Poul. , Callesen, Henrik. . 2555. "Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin".
    กรุงเทพมหานคร : Central Queensland University, Australia.
Liu, Ying. , Østrup, Olga. , Li, Juan. , Vajta, Gabor. , Lin, Lin. , Kragh, Peter M. , Purup, Stig. , Hyttel, Poul. , Callesen, Henrik. . "Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin."
    กรุงเทพมหานคร : Central Queensland University, Australia, 2555. Print.
Liu, Ying. , Østrup, Olga. , Li, Juan. , Vajta, Gabor. , Lin, Lin. , Kragh, Peter M. , Purup, Stig. , Hyttel, Poul. , Callesen, Henrik. . Increased blastocyst formation of cloned porcine embryos produced with donor cells pre-treated with Xenopus egg extract and/or digitonin. กรุงเทพมหานคร : Central Queensland University, Australia; 2555.