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Pig transgenesis by Sleeping Beauty DNA transposition

หน่วยงาน Central Queensland University, Australia

รายละเอียด

ชื่อเรื่อง : Pig transgenesis by Sleeping Beauty DNA transposition
นักวิจัย : Jakobsen, Jannik E , , Li, Juan , , Vajta, Gábor , , Bolund, Lars , , Callesen, Henrik , , Jørgensen, Arne Lund , , Nielsen, Anders Lade , , Mikkelsen, Jacob Giehm , , Kragh, Peter M , , Moldt, Brian , , Lin, Lin , , Liu, Ying , , Schmidt, Mette , , Winther, Kjeld Dahl , , Schyth, Brian Dall , , Holm, Ida E ,
คำค้น : Pig Transgenesis , Somatic Cell Nuclear Transfer , Genetic Engineering , Sleeping Beauty DNA Transposition , DNA Transposae , Systemic Gene Expression
หน่วยงาน : Central Queensland University, Australia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : http://hdl.cqu.edu.au/10018/1021358 , acquire1-20140605-144942 , cqu:11326
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Modelling of human disease in genetically engineered pigs provides unique possibilities in biomedical research and in studies of disease intervention. Establishment of methodologies that allow efficient gene insertion by non-viral gene carriers is an important step towards development of new disease models. In this report, we present transgenic pigs created by Sleeping Beauty DNA transposition in primary porcine fibroblasts in combination with somatic cell nuclear transfer by handmade cloning. Gottingen minipigs expressing green fluorescent protein are produced by transgenesis with DNA transposon vectors carrying the transgene driven by the human ubiquitin C promoter. These animals carry multiple copies (from 8 to 13) of the transgene and show systemic transgene expression. Transgene-expressing pigs carry both transposase-catalyzed insertions and at least one copy of randomly inserted plasmid DNA. Our findings illustrate critical issues related to DNA transposon-directed transgenesis, including coincidental plasmid insertion and relatively low Sleeping Beauty transposition activity in porcine fibroblasts, but also provide a platform for future development of porcine disease models using the Sleeping Beauty gene insertion technology.

บรรณานุกรม :
Jakobsen, Jannik E , , Li, Juan , , Vajta, Gábor , , Bolund, Lars , , Callesen, Henrik , , Jørgensen, Arne Lund , , Nielsen, Anders Lade , , Mikkelsen, Jacob Giehm , , Kragh, Peter M , , Moldt, Brian , , Lin, Lin , , Liu, Ying , , Schmidt, Mette , , Winther, Kjeld Dahl , , Schyth, Brian Dall , , Holm, Ida E , . "Pig transgenesis by Sleeping Beauty DNA transposition."
    กรุงเทพมหานคร : Central Queensland University, Australia, 2554. Print.