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Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls

หน่วยงาน Central Queensland University, Australia

รายละเอียด

ชื่อเรื่อง : Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls
นักวิจัย : Gao, Fei. , Luo, Yonglun. , Du, Yutao. , Yang, Huanming. , Bolund, Lars. , Li, Shengting. , Li, Jian. , Lin, Lin. , Nielsen, Anders Lade. , Sørensen, Charlotte Brandt. , Vajta, Gabor. , Wang, Jun. , Zhang, Xiuqing.
คำค้น : LIBRARY OF CONGRESS NEEDED , 970111 Expanding Knowledge in the Medical and Health Sciences. , 060499 Genetics not elsewhere classified. , Gene expression -- Cloned pigs -- In-vitro -- Somatic Cell Nuclear Transfer (SCNT) , Journal Article. Refereed, Scholarly Journal
หน่วยงาน : Central Queensland University, Australia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : http://dx.doi.org/10.1371/journal.pone.0025901 , http://hdl.cqu.edu.au/10018/1019953
ที่มา : Gao, F, Luo, Y, Li, S, Li, J, Lin, L, Nielsen, AL, Sørensen, CB, Vajta, G, Wang, J, Zhang, X, Du, Y, Yang, H & Bolund, L 2011, 'Comparison of Gene Expression and Genome-Wide DNA Methylation Profiling between Phenotypically Normal Cloned Pigs and Conventionally Bred Controls', PLoS ONE, vol. 6, no. 10, pp. e25901-e25901, http://dx.doi.org/10.1371/journal.pone.0025901
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE. USA : Public Library of Science, 2011. Vol. 6, no. 10 (October 2010), p. 1-13 13 pages Refereed 1932-6203 (online) , ACQUIRE [electronic resource] : Central Queensland University Institutional Repository.
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Animal breeding via Somatic Cell Nuclear Transfer (SCNT) has enormous potential in agriculture and biomedicine. However, concerns about whether SCNT animals are as healthy or epigenetically normal as conventionally bred ones are raised as the efficiency of cloning by SCNT is much lower than natural breeding or In-vitro fertilization (IVF). Thus, we have conducted a genome-wide gene expression and DNA methylation profiling between phenotypically normal cloned pigs and control pigs in two tissues (muscle and liver), using Affymetrix Porcine expression array as well as modified methylation-specific digital karyotyping (MMSDK) and Solexa sequencing technology. Typical tissue-specific differences with respect to both gene expression and DNA methylation were observed in muscle and liver from cloned as well as control pigs. Gene expression profiles were highly similar between cloned pigs and controls, though a small set of genes showed altered expression. Cloned pigs presented a more different pattern of DNA methylation in unique sequences in both tissues. Especially a small set of genomic sites had different DNA methylation status with a trend towards slightly increased methylation levels in cloned pigs. Molecular network analysis of the genes that contained such differential methylation loci revealed a significant network related to tissue development. In conclusion, our study showed that phenotypically normal cloned pigs were highly similar with normal breeding pigs in their gene expression, but moderate alteration in DNA methylation aspects still exists, especially in certain unique genomic regions.

บรรณานุกรม :
Gao, Fei. , Luo, Yonglun. , Du, Yutao. , Yang, Huanming. , Bolund, Lars. , Li, Shengting. , Li, Jian. , Lin, Lin. , Nielsen, Anders Lade. , Sørensen, Charlotte Brandt. , Vajta, Gabor. , Wang, Jun. , Zhang, Xiuqing. . (2554). Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls.
    กรุงเทพมหานคร : Central Queensland University, Australia.
Gao, Fei. , Luo, Yonglun. , Du, Yutao. , Yang, Huanming. , Bolund, Lars. , Li, Shengting. , Li, Jian. , Lin, Lin. , Nielsen, Anders Lade. , Sørensen, Charlotte Brandt. , Vajta, Gabor. , Wang, Jun. , Zhang, Xiuqing. . 2554. "Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls".
    กรุงเทพมหานคร : Central Queensland University, Australia.
Gao, Fei. , Luo, Yonglun. , Du, Yutao. , Yang, Huanming. , Bolund, Lars. , Li, Shengting. , Li, Jian. , Lin, Lin. , Nielsen, Anders Lade. , Sørensen, Charlotte Brandt. , Vajta, Gabor. , Wang, Jun. , Zhang, Xiuqing. . "Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls."
    กรุงเทพมหานคร : Central Queensland University, Australia, 2554. Print.
Gao, Fei. , Luo, Yonglun. , Du, Yutao. , Yang, Huanming. , Bolund, Lars. , Li, Shengting. , Li, Jian. , Lin, Lin. , Nielsen, Anders Lade. , Sørensen, Charlotte Brandt. , Vajta, Gabor. , Wang, Jun. , Zhang, Xiuqing. . Comparison of gene expression and genome-wide DNA methylation profiling between phenotypically normal cloned pigs and conventionally bred controls. กรุงเทพมหานคร : Central Queensland University, Australia; 2554.