ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Massively parallel sequencing for chromosomal abnormality testing in trophectoderm cells of human blastocysts

หน่วยงาน Central Queensland University, Australia

รายละเอียด

ชื่อเรื่อง : Massively parallel sequencing for chromosomal abnormality testing in trophectoderm cells of human blastocysts
นักวิจัย : Yin, XuYang. , Tan, Ke. , Gong, Chun. , Li, XuChao. , Lin, ChuYu. , Gao, Ya. , Liang, Yu. , Yi, Xin. , Mu, Feng. , Zhao, LiJian. , Peng, HuanHuan. , Xiong, Bo. , Zhang, ShuoPing. , Cheng, DeHua. , Lu, GuangXiu. , Zhang, Xiuqing. , Lin, Ge. , Wang, Wei. , Vajta, Gabor. , Jiang, Hui. , Tan, YueQiu. , Zhang, ChunLei. , Chen, Fang. , Chen, ShengPei. , Zhang, ChunSheng. , Pan, XiaoYu.
คำค้น : LIBRARY OF CONGRESS NEEDED , 970111 Expanding Knowledge in the Medical and Health Sciences. , 060499 Genetics not elsewhere classified. , Aneuploidy -- Blastocyst trophectoderm cells -- Massively parallel sequencing -- SNP array -- Unbalanced chromosomal rearrangement , Journal Article. Refereed, Scholarly Journal
หน่วยงาน : Central Queensland University, Australia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : http://hdl.cqu.edu.au/10018/1019814
ที่มา : Yin, X, Tan, K, Vajta, G, Jiang, H, Tan, Y, Zhang, C, Chen, F, Chen, S, Zhang, C, Pan, X, Gong, C, Li, X, Lin, C, Gao, Y, Liang, Y, Yi, X, Mu, F, Zhao, L, Peng, H, Xiong, B, Zhang, S, Cheng, D, Lu, G, Zhang, X, Lin, G & Wang, W 2013, 'Massively Parallel Sequencing for Chromosomal Abnormality Testing in Trophectoderm Cells of Human Blastocysts', Biology of Reproduction, vol. 88, no. 3, article 69, pp. 1-6, http://dx.doi.org/10.1095/biolreprod.112.106211
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biology of reproduction. USA : Society for the Study of Reproduction, 2013. Vol. 88, no. 3 (March 2013), p. 1-6 6 pages Refereed 0006-3363 1529-7268 (online) , ACQUIRE [electronic resource] : Central Queensland University Institutional Repository.
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Preimplantation genetic diagnosis and screening are widely accepted for chromosomal abnormality identification to avoid transferring embryos with genetic defects. Massively parallel sequencing (MPS) is a rapidly developing approach for genome analysis with increasing application in clinical practice. The purpose of this study was to use MPS for identification of aneuploidies and unbalanced chromosomal rearrangements after blastocyst biopsy. Trophectoderm (TE) samples of 38 blastocysts from 16 in vitro fertilization cycles were subjected to analysis. Low-coverage whole genome sequencing was performed using the Illumina HiSeq2000 platform with a novel algorithm purposely created for chromosomal analysis. The efficiency of this MPS approach was estimated by comparing results obtained by an Affymetrix single-nucleotide polymorphism (SNP) array. Whole genome amplification (WGA) products of TE cells were detected by MPS, with an averageof 0.073 depth and 5.5% coverage of the human genome. Twenty-six embryos (68.4%) were detected as euploid, while six embryos (15.8%) contained uniform aneuploidies. Four of these (10.5%) were with solely unbalanced chromosomal rearrangements, whereas the remaining two embryos (5.3%) showed both aneuploidies and unbalanced rearrangements. Almost all these results were confirmed by the SNP array, with the exception of one sample, where different sizes of unbalanced rearrangements were detected, possibly due to chromosomal GC bias in array analysis. Our study demonstrated MPS could be applied to accurately detect embryonic chromosomal abnormality with a flexible and cost-effective strategy and higher potential accuracy.

บรรณานุกรม :
Yin, XuYang. , Tan, Ke. , Gong, Chun. , Li, XuChao. , Lin, ChuYu. , Gao, Ya. , Liang, Yu. , Yi, Xin. , Mu, Feng. , Zhao, LiJian. , Peng, HuanHuan. , Xiong, Bo. , Zhang, ShuoPing. , Cheng, DeHua. , Lu, GuangXiu. , Zhang, Xiuqing. , Lin, Ge. , Wang, Wei. , Vajta, Gabor. , Jiang, Hui. , Tan, YueQiu. , Zhang, ChunLei. , Chen, Fang. , Chen, ShengPei. , Zhang, ChunSheng. , Pan, XiaoYu. . "Massively parallel sequencing for chromosomal abnormality testing in trophectoderm cells of human blastocysts."
    กรุงเทพมหานคร : Central Queensland University, Australia, 2556. Print.