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The Well-of-the-Well system an efficient approach to improve embryo development /

หน่วยงาน Central Queensland University, Australia

รายละเอียด

ชื่อเรื่อง : The Well-of-the-Well system an efficient approach to improve embryo development /
นักวิจัย : Vajta, Gabor. , Korösi, Tamás. , Du, Yutao. , Nakata, Kumiko. , Ieda, Shoko. , Kuwayama, Masashige. , Nagy, Zsolt Peter.
คำค้น : , 970111 Expanding Knowledge in the Medical and Health Sciences. , 060499 Genetics not elsewhere classified. , Blastocyst -- Culture -- Microenvironment -- Pregnancy -- Transfer , Journal Article. Refereed, Scholarly Journal
หน่วยงาน : Central Queensland University, Australia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2551
อ้างอิง : http://hdl.cqu.edu.au/10018/1021155
ที่มา : Vajta, G, Korösi, T, Du, Y, Nakata, K, Ieda, S, Kuwayama, M & Nagy, ZP 2008, 'The Well-of-the-Well system: an efficient approach to improve embryo development', Reproductive BioMedicine Online, vol. 17, no. 1, pp. 73-81, http://dx.doi.org/10.1016/S1472-6483(10)60296-9
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Reproductive biomedicine online. United Kingdom : Elsevier Ltd, 2008. Vol. 17, no. 1 (May 2008), p. 73-81 9 pages Refereed 1472-6483 1472-6491 (online) , ACQUIRE [electronic resource] : Central Queensland University Institutional Repository.
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Transfer of human embryos at the blastocyst stage may offer considerable benefits including an increased implantation rate and a decreased risk of multiple pregnancies; however, blastocyst culture requires an efficient and reliable in-vitro embryo culture system. In this study, the effect of the Well-of-the-Well (WOW) system consisting of microwells formed on the bottom of the culture dish was tested in three mammalian species, including humans. The WOW system resulted in significant improvement when comparing the drops for culture of in-vitro-matured and parthenogenetically activated porcine oocytes, and in-vivo-derived mouse zygotes. In human embryos, using a sibling oocyte design, embryos cultured in WOW developed to the blastocyst stage in a significantly higher proportion than did embryos cultured traditionally (55% in WOW and 37% in conventional culture; P < 0.05). In a separate study, also in human, a total of 48 patients with a cumulative 214 unsuccessful previous IVF cycles were selected for the trials. In subsequent intracytoplasmic sperm injection cycles, oocytes/embryos were cultured individually in the WOW system or in microdrops. Transferable quality blastocyst development (48.9% of cultured zygotes) was observed in the WOW system. Ninety-four blastocysts transferred to 45 patients resulted in clinical pregnancy rates of 48.9%, including nine twin pregnancies, seven single pregnancies, five miscarriages and one ectopic pregnancy. The results indicate that the WOW system provides a promising alternative for microdrop culture of mammalian embryos, including human embryos.

บรรณานุกรม :
Vajta, Gabor. , Korösi, Tamás. , Du, Yutao. , Nakata, Kumiko. , Ieda, Shoko. , Kuwayama, Masashige. , Nagy, Zsolt Peter. . (2551). The Well-of-the-Well system an efficient approach to improve embryo development /.
    กรุงเทพมหานคร : Central Queensland University, Australia.
Vajta, Gabor. , Korösi, Tamás. , Du, Yutao. , Nakata, Kumiko. , Ieda, Shoko. , Kuwayama, Masashige. , Nagy, Zsolt Peter. . 2551. "The Well-of-the-Well system an efficient approach to improve embryo development /".
    กรุงเทพมหานคร : Central Queensland University, Australia.
Vajta, Gabor. , Korösi, Tamás. , Du, Yutao. , Nakata, Kumiko. , Ieda, Shoko. , Kuwayama, Masashige. , Nagy, Zsolt Peter. . "The Well-of-the-Well system an efficient approach to improve embryo development /."
    กรุงเทพมหานคร : Central Queensland University, Australia, 2551. Print.
Vajta, Gabor. , Korösi, Tamás. , Du, Yutao. , Nakata, Kumiko. , Ieda, Shoko. , Kuwayama, Masashige. , Nagy, Zsolt Peter. . The Well-of-the-Well system an efficient approach to improve embryo development /. กรุงเทพมหานคร : Central Queensland University, Australia; 2551.