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Handmade cloned transgenic sheep rich in omega-3 fatty acids

หน่วยงาน Central Queensland University, Australia

รายละเอียด

ชื่อเรื่อง : Handmade cloned transgenic sheep rich in omega-3 fatty acids
นักวิจัย : Zhang, Peng. , Liu, Peng. , Ma, Runlin Z. , Dou, Hongwei. , Chen, Lei. , Chen, Longxin. , Lin, Lin. , Tan, Pingping. , Vajta, Gabor. , Gao, Jianfeng. , Du, Yutao.
คำค้น : LIBRARY OF CONGRESS NEEDED , 970111 Expanding Knowledge in the Medical and Health Sciences. , 060499 Genetics not elsewhere classified. , Transgenic sheep -- Omega-3 fatty acids -- Handmade cloning , Journal Article. Refereed, Scholarly Journal
หน่วยงาน : Central Queensland University, Australia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : http://dx.doi.org/10.1371/journal.pone.0055941 , http://hdl.cqu.edu.au/10018/1019938
ที่มา : Zhang, P, Liu, P, Dou, H, Chen, L, Chen, L, Lin, L, Tan, P, Vajta, G, Gao, J, Du, Y & Ma, RZ 2013, 'Handmade Cloned Transgenic Sheep Rich in Omega-3 Fatty Acids', PLoS ONE, vol. 8, no. 2, pp. e55941-e55941, http://dx.doi.org/10.1371/journal.pone.0055941
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE. USA : Public Library of Science, 2013. Vol. 8, no. 2 (February 2013), p. 1-9 9 pages Refereed 1932-6203 (online) , ACQUIRE [electronic resource] : Central Queensland University Institutional Repository.
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Technology of somatic cell nuclear transfer (SCNT) has been adapted worldwide to generate transgenic animals, although the traditional procedure relies largely on instrumental micromanipulation. In this study, we used the modified handmade cloning (HMC) established in cattle and pig to produce transgenic sheep with elevated levels of omega-3 (n-3) fatty acids. Codon-optimized nematode mfat-1 was inserted into a eukaryotic expression vector and was transferred into the genome of primary ovine fibroblast cells from a male Chinese merino sheep. Reverse transcriptase PCR, gas chromatography, and chromosome analyses were performed to select nuclear donor cells capable of converting omega-6 (n-6) into n-3 fatty acids. Blastocysts developed after 7 days of in vitro culture were surgically transplanted into the uterus of female ovine recipients of a local sheep breed in Xinjiang. For the HMC, approximately 8.9% (n = 925) of reconstructed embryos developed to the blastocyst stage. Four recipients became pregnant after 53 blastocysts were transplanted into 29 naturally cycling females, and a total of 3 live transgenic lambs were produced. Detailed analyses on one of the transgenic lambs revealed a single integration of the modified nematode mfat-1 gene at sheep chromosome 5. The transgenic sheep expressed functional n23 fatty acid desaturase, accompanied by more than 2-folds reduction of n-6/n-3 ratio in the muscle (p<0.01) and other major organs/tissues (p<0.05). To our knowledge, this is the first report of transgenic sheep produced by the HMC. Compared to the traditional SCNT method, HMC showed an equivalent efficiency but proved cheaper and easier in operation.

บรรณานุกรม :
Zhang, Peng. , Liu, Peng. , Ma, Runlin Z. , Dou, Hongwei. , Chen, Lei. , Chen, Longxin. , Lin, Lin. , Tan, Pingping. , Vajta, Gabor. , Gao, Jianfeng. , Du, Yutao. . (2556). Handmade cloned transgenic sheep rich in omega-3 fatty acids.
    กรุงเทพมหานคร : Central Queensland University, Australia.
Zhang, Peng. , Liu, Peng. , Ma, Runlin Z. , Dou, Hongwei. , Chen, Lei. , Chen, Longxin. , Lin, Lin. , Tan, Pingping. , Vajta, Gabor. , Gao, Jianfeng. , Du, Yutao. . 2556. "Handmade cloned transgenic sheep rich in omega-3 fatty acids".
    กรุงเทพมหานคร : Central Queensland University, Australia.
Zhang, Peng. , Liu, Peng. , Ma, Runlin Z. , Dou, Hongwei. , Chen, Lei. , Chen, Longxin. , Lin, Lin. , Tan, Pingping. , Vajta, Gabor. , Gao, Jianfeng. , Du, Yutao. . "Handmade cloned transgenic sheep rich in omega-3 fatty acids."
    กรุงเทพมหานคร : Central Queensland University, Australia, 2556. Print.
Zhang, Peng. , Liu, Peng. , Ma, Runlin Z. , Dou, Hongwei. , Chen, Lei. , Chen, Longxin. , Lin, Lin. , Tan, Pingping. , Vajta, Gabor. , Gao, Jianfeng. , Du, Yutao. . Handmade cloned transgenic sheep rich in omega-3 fatty acids. กรุงเทพมหานคร : Central Queensland University, Australia; 2556.