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Cell Colony Formation Induced by Xenopus Egg Extract as a Marker for Improvement of Cloned Blastocyst Formation in the Pig

หน่วยงาน Central Queensland University, Australia

รายละเอียด

ชื่อเรื่อง : Cell Colony Formation Induced by Xenopus Egg Extract as a Marker for Improvement of Cloned Blastocyst Formation in the Pig
นักวิจัย : Liu, Ying , , Østrup, Olga , , Li, Juan , , Vajta, Gabor. , , Kragh, Peter M. , , Purup, Stig , , Callesen, Henrik ,
คำค้น : Na
หน่วยงาน : Central Queensland University, Australia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : http://hdl.cqu.edu.au/10018/1019769 , acquire1-20140411-100429 , cqu:11130
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Treatment with cytoplasmic extracts from Xenopus laevis eggs represents a potential tool for universal cellular reprogramming. However, the biochemical activity and quality of the extract vary from batch to batch. This study aimed to evaluate three different extract batches prepared by the same method based on the colony formation of cells after extract treatment, and subsequent in vitro cloning efficiency using treated cells as chromatin donors. Porcine fetal fibroblasts were treated with each batch of extract, and cultured in embryonic stem cell (ES) medium for 12 days. The number of forming colonies in treated cells was counted on Day 7 after extract treatment and significant variability was detected between different batches of extract. Similarly, when using cells from colonies at Days 7 to 8 after treatment for handmade cloning, increased blastocyst formation rates were observed after the cells were treated with a batch showing higher colony formation. In conclusion, assessment of cell colony formation may be used as selection marker for Xenopus egg extract used for pretreatment of donor cells prior to cloning.

บรรณานุกรม :
Liu, Ying , , Østrup, Olga , , Li, Juan , , Vajta, Gabor. , , Kragh, Peter M. , , Purup, Stig , , Callesen, Henrik , . (2554). Cell Colony Formation Induced by Xenopus Egg Extract as a Marker for Improvement of Cloned Blastocyst Formation in the Pig.
    กรุงเทพมหานคร : Central Queensland University, Australia.
Liu, Ying , , Østrup, Olga , , Li, Juan , , Vajta, Gabor. , , Kragh, Peter M. , , Purup, Stig , , Callesen, Henrik , . 2554. "Cell Colony Formation Induced by Xenopus Egg Extract as a Marker for Improvement of Cloned Blastocyst Formation in the Pig".
    กรุงเทพมหานคร : Central Queensland University, Australia.
Liu, Ying , , Østrup, Olga , , Li, Juan , , Vajta, Gabor. , , Kragh, Peter M. , , Purup, Stig , , Callesen, Henrik , . "Cell Colony Formation Induced by Xenopus Egg Extract as a Marker for Improvement of Cloned Blastocyst Formation in the Pig."
    กรุงเทพมหานคร : Central Queensland University, Australia, 2554. Print.
Liu, Ying , , Østrup, Olga , , Li, Juan , , Vajta, Gabor. , , Kragh, Peter M. , , Purup, Stig , , Callesen, Henrik , . Cell Colony Formation Induced by Xenopus Egg Extract as a Marker for Improvement of Cloned Blastocyst Formation in the Pig. กรุงเทพมหานคร : Central Queensland University, Australia; 2554.