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Handmade cloned transgenic piglets expressing the nematode fat-1 gene

หน่วยงาน Central Queensland University, Australia

รายละเอียด

ชื่อเรื่อง : Handmade cloned transgenic piglets expressing the nematode fat-1 gene
นักวิจัย : Zhang, Peng. , Zhang, Yidi. , Dou, Hongwei. , Yin, Jingdong. , Chen, Yu. , Pang, Xinzhi. , Vajta, Gabor. , Bolund, Lars. , Du, Yutao. , Ma, Runlin Z.
คำค้น : 970111 Expanding Knowledge in the Medical and Health Sciences. , 060499 Genetics not elsewhere classified. , Transgenic pigs -- Handmade cloning , Journal Article. Refereed, Scholarly Journal
หน่วยงาน : Central Queensland University, Australia
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : http://hdl.cqu.edu.au/10018/1019665
ที่มา : Zhang, P, Zhang, Y, Dou, H, Yin, J, Chen, Y, Pang, X, Vajta, G, Bolund, L, Du, Y & Ma, RZ 2012, 'Handmade Cloned Transgenic Piglets Expressing the Nematode Fat-1 Gene', Cellular Reprogramming, vol. 14, no.3, pp. 258-266, http://dx.doi.org/10.1089/cell.2011.0073
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Cellular reprogramming. USA : Mary Ann Liebert, 2012. Vol. 14, no.3 (June 2012), p. 258-266 9 pages Refereed 2152-4971 2152-4998 (online) , ACQUIRE [electronic resource] : Central Queensland University Institutional Repository.
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Production of transgenic animals via somatic cell nuclear transfer (SCNT) has been adapted worldwide, but this application is somewhat limited by its relatively low efficiency. In this study, we used handmade cloning (HMC) established previously to produce transgenic pigs that express the functional nematode fat-1 gene. Codon-optimized mfat-1 was inserted into eukaryotic expression vectors, which were transferred into primary swine donor cells. Reverse transcriptase PCR (RT-PCR), gas chromatography, and chromosome analyses were performed to select donor clones capable of converting n-6 into n-3 fatty acids. Blastocysts derived from the clones that lowered the n-6/n-3 ratio to approximately 1:1 were transferred surgically into the uteri of recipients for transgenic piglets. By HMC, 37% (n = 558) of reconstructed embryos developed to the blastocyst stage after 7 days of culture in vitro, with an average cell number of 81 – 36 (n = 14). Three recipients became pregnant after 408 day-6 blastocysts were transferred into four naturally cycling females, and a total of 14 live offspring were produced. The nematode mfat-1 effectively lowered the n-6/n-3 ratio in muscle and major organs of the transgenic pig. Our results will help to establish a reliable procedure and an efficient option in the production of transgenic animals.

บรรณานุกรม :
Zhang, Peng. , Zhang, Yidi. , Dou, Hongwei. , Yin, Jingdong. , Chen, Yu. , Pang, Xinzhi. , Vajta, Gabor. , Bolund, Lars. , Du, Yutao. , Ma, Runlin Z. . (2555). Handmade cloned transgenic piglets expressing the nematode fat-1 gene.
    กรุงเทพมหานคร : Central Queensland University, Australia.
Zhang, Peng. , Zhang, Yidi. , Dou, Hongwei. , Yin, Jingdong. , Chen, Yu. , Pang, Xinzhi. , Vajta, Gabor. , Bolund, Lars. , Du, Yutao. , Ma, Runlin Z. . 2555. "Handmade cloned transgenic piglets expressing the nematode fat-1 gene".
    กรุงเทพมหานคร : Central Queensland University, Australia.
Zhang, Peng. , Zhang, Yidi. , Dou, Hongwei. , Yin, Jingdong. , Chen, Yu. , Pang, Xinzhi. , Vajta, Gabor. , Bolund, Lars. , Du, Yutao. , Ma, Runlin Z. . "Handmade cloned transgenic piglets expressing the nematode fat-1 gene."
    กรุงเทพมหานคร : Central Queensland University, Australia, 2555. Print.
Zhang, Peng. , Zhang, Yidi. , Dou, Hongwei. , Yin, Jingdong. , Chen, Yu. , Pang, Xinzhi. , Vajta, Gabor. , Bolund, Lars. , Du, Yutao. , Ma, Runlin Z. . Handmade cloned transgenic piglets expressing the nematode fat-1 gene. กรุงเทพมหานคร : Central Queensland University, Australia; 2555.