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Effects of polycaprolactone-based scaffolds on the blood-brain barrier and cerebral inflammation

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Effects of polycaprolactone-based scaffolds on the blood-brain barrier and cerebral inflammation
นักวิจัย : Nga, Vincent Diong Weng , Lim, Jing , Choy, David Kim Seng , Nyein, Mya Aye , Lu, Jia , Chou, Ning , Yeo, Tseng Tsai , Teoh, Swee-Hin
คำค้น : DRNTU::Science::Medicine::Tissue engineering
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2558
อ้างอิง : Nga, V. D. W., Lim, J., Choy, D. K. S., Nyein, M. A., Lu, J., Chou, N., et al. (2015). Effects of polycaprolactone-based scaffolds on the blood-brain barrier and cerebral inflammation. Tissue engineering part A, 21(3-4), 647-653. , 1937-3341 , http://hdl.handle.net/10220/25229 , http://dx.doi.org/10.1089/ten.tea.2013.0779
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Tissue engineering part A
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Severe pathoanatomical and mechanical injuries compromise patient recovery and survival following penetrating brain injury (PBI). The realization that the blood–brain barrier (BBB) plays a major role in dictating post-PBI events has led to rising interests in possible therapeutic interventions through the BBB. Recently, the choroid plexus has also been suggested as a potential therapeutic target. The use of biocompatible scaffolds for the delivery of therapeutic agents, but little is known about their interaction with cerebral tissue, which has important clinical implications. Therefore, the authors have sought to investigate the effect of polycaprolactone (PCL) and PCL/tricalcium phosphate (PCL/TCP) scaffolds on the maintenance of BBB phenotype posttraumatic brain injury. Cranial defects of 3 mm depth were created in Sprague Dawley rats, and PCL and PCL/TCP scaffolds were subsequently implanted in predetermined locations for a period of 1 week and 1 month. Higher endothelial barrier antigen (EBA) expressions from PCL-based scaffold groups (p>0.05) were found, suggesting slight advantages over the sham group (no scaffold implantation). PCL/TCP scaffold group also expressed EBA to a higher degree (p>0.05) than PCL scaffolds. Importantly, higher capillary count and area as early as 1 week postimplantation suggested lowered ischemia from the PCL/TCP scaffold group as compared with PCL and sham. Evaluation of interlukin-1β expression suggested that the PCL and PCL/TCP scaffolds did not cause prolonged inflammation. BBB transport selectivity was evaluated by the expression of aquaporin-4 (AQP-4). Attenuated expression of AQP-4 in the PCL/TCP group (p<0.05) suggested that PCL/TCP scaffolds altered BBB selectivity to a lower degree as compared with sham and PCL groups, pointing to potential clinical implications in reducing cerebral edema. Taken together, the responses of PCL-based scaffolds with brain tissue suggested safety, and encourages further preclinical evaluation in PBI management with these scaffolds.

บรรณานุกรม :
Nga, Vincent Diong Weng , Lim, Jing , Choy, David Kim Seng , Nyein, Mya Aye , Lu, Jia , Chou, Ning , Yeo, Tseng Tsai , Teoh, Swee-Hin . (2558). Effects of polycaprolactone-based scaffolds on the blood-brain barrier and cerebral inflammation.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Nga, Vincent Diong Weng , Lim, Jing , Choy, David Kim Seng , Nyein, Mya Aye , Lu, Jia , Chou, Ning , Yeo, Tseng Tsai , Teoh, Swee-Hin . 2558. "Effects of polycaprolactone-based scaffolds on the blood-brain barrier and cerebral inflammation".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Nga, Vincent Diong Weng , Lim, Jing , Choy, David Kim Seng , Nyein, Mya Aye , Lu, Jia , Chou, Ning , Yeo, Tseng Tsai , Teoh, Swee-Hin . "Effects of polycaprolactone-based scaffolds on the blood-brain barrier and cerebral inflammation."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2558. Print.
Nga, Vincent Diong Weng , Lim, Jing , Choy, David Kim Seng , Nyein, Mya Aye , Lu, Jia , Chou, Ning , Yeo, Tseng Tsai , Teoh, Swee-Hin . Effects of polycaprolactone-based scaffolds on the blood-brain barrier and cerebral inflammation. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2558.