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Redox-responsive mesoporous silica nanoparticles : a physiologically sensitive codelivery vehicle for siRNA and doxorubicin

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Redox-responsive mesoporous silica nanoparticles : a physiologically sensitive codelivery vehicle for siRNA and doxorubicin
นักวิจัย : Ma, Xing , Teh, Cathleen , Zhang, Quan , Borah, Parijat , Choong, Cleo , Korzh, Vladimir , Zhao, Yanli
คำค้น : DRNTU::Science::Medicine
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Ma, X., Teh, C., Zhang, Q., Borah, P., Choong, C., Korzh, V., et al. (2014). Redox-responsive mesoporous silica nanoparticles : a physiologically sensitive codelivery vehicle for siRNA and doxorubicin. Antioxidants & redox signaling, 21(5), 707-722. , 1523-0864 , http://hdl.handle.net/10220/25156 , http://dx.doi.org/10.1089/ars.2012.5076
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Antioxidants & redox signaling
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Aims: Efficient siRNA/drug codelivery carriers can offer great promises to cancer treatment on account of synergistic effect provided from cancer-associated gene and anticancer drugs. In this work, a redox-responsive drug/siRNA codelivery vehicle based on mesoporous silica nanoparticles was fabricated to simultaneously deliver siRNA and doxorubicin (Dox) in vitro and in vivo. Results: The nanoparticle surface was functionalized with the adamantane (AD) units. Formation of stable host–guest complex between disulfide bond linked-AD and ethylenediamine-modified β-cyclodextrin is capable of fully blocking drugs inside the nanopores, while amino groups can complex with siRNA via electrostatic interaction. Relatively high concentration of glutathione in biophysical environment provides natural reducing agent to trigger drug/siRNA release by cleaving pre-introduced disulfide bonds. B-cell lymphoma 2 (Bcl-2) siRNA was codelivered to silence Bcl-2 protein expression in HeLa cells, resulting in enhanced chemotherapy efficacy in vitro. In vivo delivery experiment carried out in transgenic zebrafish larvae indicates that the delivery of Dox inhibits the development of choroid plexus in a dose-dependent manner, leading to successful decrease of green fluorescence protein transcription in choroid plexus. Reduction of liver tumor was also demonstrated after injection of Dox-loaded nanoparticles. Innovation: We successfully demonstrated that functional nanoparticles could serve as an efficient carrier for the delivery of Bcl-2 siRNA and Dox in HeLa cells and in transgenic zebrafish larvae, leading to enhanced therapeutic efficacy. Conclusion: Enhanced cytotoxicity caused by simultaneous delivery of Bcl-2 siRNA and Dox was observed in HeLa cells. Drug-loaded nanoparticles were internalized in vivo, inhibiting the development of choroid plexus and the progression of liver tumor.

บรรณานุกรม :
Ma, Xing , Teh, Cathleen , Zhang, Quan , Borah, Parijat , Choong, Cleo , Korzh, Vladimir , Zhao, Yanli . (2557). Redox-responsive mesoporous silica nanoparticles : a physiologically sensitive codelivery vehicle for siRNA and doxorubicin.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ma, Xing , Teh, Cathleen , Zhang, Quan , Borah, Parijat , Choong, Cleo , Korzh, Vladimir , Zhao, Yanli . 2557. "Redox-responsive mesoporous silica nanoparticles : a physiologically sensitive codelivery vehicle for siRNA and doxorubicin".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ma, Xing , Teh, Cathleen , Zhang, Quan , Borah, Parijat , Choong, Cleo , Korzh, Vladimir , Zhao, Yanli . "Redox-responsive mesoporous silica nanoparticles : a physiologically sensitive codelivery vehicle for siRNA and doxorubicin."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Ma, Xing , Teh, Cathleen , Zhang, Quan , Borah, Parijat , Choong, Cleo , Korzh, Vladimir , Zhao, Yanli . Redox-responsive mesoporous silica nanoparticles : a physiologically sensitive codelivery vehicle for siRNA and doxorubicin. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.