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Evaluation of In Vitro Cross-Reactivity to Avian {H5N1} and Pandemic {H1N1} 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial

หน่วยงาน สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ

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ชื่อเรื่อง : Evaluation of In Vitro Cross-Reactivity to Avian {H5N1} and Pandemic {H1N1} 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial
นักวิจัย : Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul
คำค้น : Antibodies , Booster doses , H5N1 , Influenza , Influenza viruses , Vaccines , สาขาวิทยาศาสตร์การแพทย์
หน่วยงาน : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : http://dx.doi.org/10.1371/journal.pone.0059674 , http://www.nstda.or.th/thairesearch/node/24958
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

{IntroductionRecent} studies have demonstrated that inactivated seasonal influenza vaccines ({IIV)} may elicit production of heterosubtypic antibodies, which can neutralize avian {H5N1} virus in a small proportion of subjects. We hypothesized that prime boost regimens of live and inactivated trivalent seasonal influenza vaccines ({LAIV} and {IIV)} would enhance production of heterosubtypic immunity and provide evidence of cross-protection against other influenza {viruses.MethodsIn} an open-label study, 26 adult volunteers were randomized to receive one of four vaccine regimens containing two doses of 2009-10 seasonal influenza vaccines administered 8 (±1) weeks apart: 2 doses of {LAIV;} 2 doses of {IIV;} {LAIV} then {IIV;} {IIV} then {LAIV.} Humoral immunity assays for avian {H5N1}, 2009 pandemic {H1N1} ({pH1N1)}, and seasonal vaccine strains were performed on blood collected pre-vaccine and 2 and 4 weeks later. The percentage of cytokine-producing T-cells was compared with baseline 14 days after each {dose.ResultsSubjects} receiving {IIV} had prompt serological responses to vaccine strains. Two subjects receiving heterologous prime boost regimens had enhanced haemagglutination inhibition ({HI)} and neutralization ({NT)} titres against {pH1N1}, and one subject against avian {H5N1;} all three had pre-existing cross-reactive antibodies detected at baseline. Significantly elevated titres to {H5N1} and {pH1N1} by neuraminidase inhibition ({NI)} assay were observed following {LAIV-IIV} administration. Both vaccines elicited cross-reactive {CD4+} T-cell responses to nucleoprotein of avian {H5N1} and {pH1N1.} All regimens were safe and well {tolerated.ConclusionNeither} homologous nor heterologous prime boost immunization enhanced serum {HI} and {NT} titres to 2009 {pH1N1} or avian {H5N1} compared to single dose vaccine. However heterologous prime-boost vaccination did lead to in vitro evidence of cross-reactivity by {NI;} the significance of this finding is unclear. These data support the strategy of administering single dose trivalent seasonal influenza vaccine at the outset of an influenza pandemic while a specific vaccine is being {developed.Trial} {RegistrationClinicalTrials.gov} {NCT01044095}

บรรณานุกรม :
Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul . (2556). Evaluation of In Vitro Cross-Reactivity to Avian {H5N1} and Pandemic {H1N1} 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial.
    ปทุมธานี : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ.
Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul . 2556. "Evaluation of In Vitro Cross-Reactivity to Avian {H5N1} and Pandemic {H1N1} 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial".
    ปทุมธานี : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ.
Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul . "Evaluation of In Vitro Cross-Reactivity to Avian {H5N1} and Pandemic {H1N1} 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial."
    ปทุมธานี : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ, 2556. Print.
Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul . Evaluation of In Vitro Cross-Reactivity to Avian {H5N1} and Pandemic {H1N1} 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial. ปทุมธานี : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ; 2556.