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Evaluation of In Vitro Cross-Reactivity to Avian H5N1 and Pandemic H1N1 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial

หน่วยงาน สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ

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ชื่อเรื่อง : Evaluation of In Vitro Cross-Reactivity to Avian H5N1 and Pandemic H1N1 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial
นักวิจัย : Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul
คำค้น : antibody production , antigenic variation , H1N1 influenza , Influenza A virus , virus antibody , virus antigen , สาขาวิทยาศาสตร์การแพทย์
หน่วยงาน : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : http://www.nstda.or.th/thairesearch/node/24835
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

IntroductionRecent studies have demonstrated that inactivated seasonal influenza vaccines (IIV) may elicit production of heterosubtypic antibodies, which can neutralize avian H5N1 virus in a small proportion of subjects. We hypothesized that prime boost regimens of live and inactivated trivalent seasonal influenza vaccines (LAIV and IIV) would enhance production of heterosubtypic immunity and provide evidence of cross-protection against other influenza viruses.MethodsIn an open-label study, 26 adult volunteers were randomized to receive one of four vaccine regimens containing two doses of 2009-10 seasonal influenza vaccines administered 8 (±1) weeks apart: 2 doses of LAIV; 2 doses of IIV; LAIV then IIV; IIV then LAIV. Humoral immunity assays for avian H5N1, 2009 pandemic H1N1 (pH1N1), and seasonal vaccine strains were performed on blood collected pre-vaccine and 2 and 4 weeks later. The percentage of cytokine-producing T-cells was compared with baseline 14 days after each dose.ResultsSubjects receiving IIV had prompt serological responses to vaccine strains. Two subjects receiving heterologous prime boost regimens had enhanced haemagglutination inhibition (HI) and neutralization (NT) titres against pH1N1, and one subject against avian H5N1; all three had pre-existing cross-reactive antibodies detected at baseline. Significantly elevated titres to H5N1 and pH1N1 by neuraminidase inhibition (NI) assay were observed following LAIV-IIV administration. Both vaccines elicited cross-reactive CD4+ T-cell responses to nucleoprotein of avian H5N1 and pH1N1. All regimens were safe and well tolerated.ConclusionNeither homologous nor heterologous prime boost immunization enhanced serum HI and NT titres to 2009 pH1N1 or avian H5N1 compared to single dose vaccine. However heterologous prime-boost vaccination did lead to in vitro evidence of cross-reactivity by NI; the significance of this finding is unclear. These data support the strategy of administering single dose trivalent seasonal influenza vaccine at the outset of an influenza pandemic while a specific vaccine is being developed.Trial RegistrationClinicalTrials.gov NCT01044095

บรรณานุกรม :
Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul . (2556). Evaluation of In Vitro Cross-Reactivity to Avian H5N1 and Pandemic H1N1 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial.
    ปทุมธานี : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ.
Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul . 2556. "Evaluation of In Vitro Cross-Reactivity to Avian H5N1 and Pandemic H1N1 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial".
    ปทุมธานี : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ.
Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul . "Evaluation of In Vitro Cross-Reactivity to Avian H5N1 and Pandemic H1N1 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial."
    ปทุมธานี : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ, 2556. Print.
Bethell, Delia , Saunders, David , Anan Jongkaewwattana , Jarin Kramyu , Thitithayanont, Arunee , Suwimon Wiboon-ut , Kosol Yongvanitchit , Amporn Limsalakpetch , Utaiwan Kum-Arb , Uthaimongkol, Nichapat , Garcia, Jean Michel , Timmermans, Ans E. , Peiris, Malik , Thomas, Stephen , Engering, Anneke , Jarman, Richard G. , Mongkolsirichaikul, Duangrat , Mason, Carl , Khemnu, Nuanpan , Tyner, Stuart D. , Mark M. Fukuda , Walsh, Douglas S. , Sathit Pichyangkul . Evaluation of In Vitro Cross-Reactivity to Avian H5N1 and Pandemic H1N1 2009 Influenza Following Prime Boost Regimens of Seasonal Influenza Vaccination in Healthy Human Subjects: A Randomised Trial. ปทุมธานี : สำนักงานพัฒนาวิทยาศาสตร์และเทคโนโลยีแห่งชาติ; 2556.