ชื่อเรื่อง | : | CD14-/CD34+ is the founding population of umbilical cord blood-derived endothelial progenitor cells and angiogenin1 is an important factor promoting the colony formation |
นักวิจัย | : | Sakchai Sudchada , Pakpoom Kheolamai , Yaowalak U-Pratya , Methichit Chayosumrit , Aungkura Supokawej , Sirikul Manochantr , Chairat Tantrawatpan , Hathaitip Sritanaudomchai , Surapol Issaragrisil |
คำค้น | : | Angiogenin1 , CD14-/CD34+ , Colony formation , Cytokines , Endothelial progenitor cell , Umbilical cord blood , Stem cells , Angiogenesis |
หน่วยงาน | : | สถาบันวิจัยและให้คำปรึกษาแห่ง มหาวิทยาลัยธรรมศาสตร์ |
ผู้ร่วมงาน | : | - |
ปีพิมพ์ | : | 2555 |
อ้างอิง | : | Annals of hematology. 91,3 (2012) pp. 321-329 , 0939-5555 , http://dspace.library.tu.ac.th/handle/3517/6511 |
ที่มา | : | - |
ความเชี่ยวชาญ | : | - |
ความสัมพันธ์ | : | - |
ขอบเขตของเนื้อหา | : | - |
บทคัดย่อ/คำอธิบาย | : | The origin of endothelial progenitor cells (EPCs) in umbilical cord blood (UCB) is unknown. In this study, we explored the origin of UCB-derived EPCs by culturing CD14+ or CD14- subpopulation separately and co-culturing these two subpopulations either with or without transwells. We found no colony formation with CD14+ or CD14- subpopulation alone, but there were EPC colonies observed in direct co-cultures of both subpopulations. Transwell culture system was used to further study the effect of cytokines on EPC colony formation. We observed the presence of EPC colonies derived from CD14- subpopulation in the presence of CD14+ subpopulation in the upper compartment whereas there was no colony generated from CD14+ subpopulation with CD14- subpopulation in the upper compartment. Therefore, CD14- subpopulation is likely to be the origin of EPCs and EPC colony derivation requires cytokines released from CD14+ subpopulation. We further characterized the founding population of UCB-derived EPCs by separating CD14- subpopulation into CD14-/CD34+ and CD14-/CD34- subpopulations. There were colonies observed only in co-cultures of CD14+ with CD14-/CD34+ subpopulation but not with CD14-/CD34- subpopulation either with or without transwells. We screened 42 cytokines involving in angiogenesis using an ELISA array in the supernatant collected from CD14+ compared to CD14- subpopulations. We found consistently the presence of angiogenin1 in the supernatant of CD14+ subpopulation but not in that of CD14- subpopulation. The addition of angiogenin1 in culture of CD14- subpopulation yielded EPC colonies. We conclude that UCB-derived EPCs are confined to CD14-/CD34+ subpopulation and angiogenin1 released from CD14+ subpopulation may be an important factor promoting the EPC colony formation. © 2011 Springer-Verlag. |
บรรณานุกรม | : |
Sakchai Sudchada , Pakpoom Kheolamai , Yaowalak U-Pratya , Methichit Chayosumrit , Aungkura Supokawej , Sirikul Manochantr , Chairat Tantrawatpan , Hathaitip Sritanaudomchai , Surapol Issaragrisil . (2555). CD14-/CD34+ is the founding population of umbilical cord blood-derived endothelial progenitor cells and angiogenin1 is an important factor promoting the colony formation.
กรุงเทพมหานคร : สถาบันวิจัยและให้คำปรึกษาแห่ง มหาวิทยาลัยธรรมศาสตร์ . Sakchai Sudchada , Pakpoom Kheolamai , Yaowalak U-Pratya , Methichit Chayosumrit , Aungkura Supokawej , Sirikul Manochantr , Chairat Tantrawatpan , Hathaitip Sritanaudomchai , Surapol Issaragrisil . 2555. "CD14-/CD34+ is the founding population of umbilical cord blood-derived endothelial progenitor cells and angiogenin1 is an important factor promoting the colony formation".
กรุงเทพมหานคร : สถาบันวิจัยและให้คำปรึกษาแห่ง มหาวิทยาลัยธรรมศาสตร์ . Sakchai Sudchada , Pakpoom Kheolamai , Yaowalak U-Pratya , Methichit Chayosumrit , Aungkura Supokawej , Sirikul Manochantr , Chairat Tantrawatpan , Hathaitip Sritanaudomchai , Surapol Issaragrisil . "CD14-/CD34+ is the founding population of umbilical cord blood-derived endothelial progenitor cells and angiogenin1 is an important factor promoting the colony formation."
กรุงเทพมหานคร : สถาบันวิจัยและให้คำปรึกษาแห่ง มหาวิทยาลัยธรรมศาสตร์ , 2555. Print. Sakchai Sudchada , Pakpoom Kheolamai , Yaowalak U-Pratya , Methichit Chayosumrit , Aungkura Supokawej , Sirikul Manochantr , Chairat Tantrawatpan , Hathaitip Sritanaudomchai , Surapol Issaragrisil . CD14-/CD34+ is the founding population of umbilical cord blood-derived endothelial progenitor cells and angiogenin1 is an important factor promoting the colony formation. กรุงเทพมหานคร : สถาบันวิจัยและให้คำปรึกษาแห่ง มหาวิทยาลัยธรรมศาสตร์ ; 2555.
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