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Identification of acinetobacter species isolated from clinical specimen by photobiotin labelling DNA-DNA hybridization

หน่วยงาน จุฬาลงกรณ์มหาวิทยาลัย

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ชื่อเรื่อง : Identification of acinetobacter species isolated from clinical specimen by photobiotin labelling DNA-DNA hybridization
นักวิจัย : Chutima Sereekul
คำค้น : DNA , DNA fingerprinting , Hybridization , Acinetobacter
หน่วยงาน : จุฬาลงกรณ์มหาวิทยาลัย
ผู้ร่วมงาน : Somboon Tanasupawat , Chulalongkorn University. Graduate School
ปีพิมพ์ : 2544
อ้างอิง : 9740316581 , http://cuir.car.chula.ac.th/handle/123456789/4266
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Thesis (M.Sc.)--Chulalongkorn University, 2001

Identification of a total 350 rod-shaped gram-negative bacterial isolates, obtained from King Chulalongkorn Memorial Hospital (75 isolates) and Siriraj Hospital (275 isolates) was performed. One hundred-and fifty isolates were grouped as Acinetobacter by the presumptive phenotypic characteristics. Their phenotypic characteristics, Vitek 32 system, DNA relatedness and ubiquinone components of the isolates were carried out. On the basis of the DNA relatedness, the organisms were separated into 7 groups. One-hundred and five isolates (Group I) showed DNA homologies more than 70.11% with A.baumannii JMC 6841T. They were identified as A.baumannii. These isolates oxidized glucose, grew at 44 ํC and produced acid from glucose. The strains tested contained the major ubiquinone with 9 isoprene units (Q-9). Five isolates (Group II) showed DNA homologies more than 71.22% with A.calcoaceticus DMST 2270T. They were identified as A.calcoaceticus. They could not grow at 41 ํC. The strain tested had Q-8 as a major ubiquinone component. Two isolates (Group II) showed DNA homologies more than 70.33% compared to A.Iwoffii JCM 6840T. They were identified as A.Iwoffii. They could not oxidize glucose, not utilize L-arginine, and citrate and no growth at 41 ํC. The strain tested contained Q-9 as a major ubiquinone component. Ten isolates (Group IV) showed DNA homologies more than 72.08% with A.junii DMST 2274T. They were identified as A.junii. These isolates could not oxidize glucose but could utilize L-arginine and citrate and some tested strains grew at 41 ํC. The strain tested had Q-9 as a major ubiquinone component. Four isolates (Group V) showed DNA homologies more than 82.45% with A.haemolyticus DMST 2273T and they were identified as A.haemolyticus. The isolated (Group VI) showed DNA homologies more than 81.22% with Acinetobacter genospecies 3 DMST 2272T. They were identified as Acinetobacter genospecies 3. They could oxidize glucose and grow at 41 ํC. The strains tested contained Q-9 as a major ubiquinone component. Twenty-one isolates (Group VII) showed DNA homologies of less than 10.0-69.67% with all the 9 type strains of Acinetobacter species including A.baumannii JMC 6841T, A.calcoaceticus DMST 2270T, A.Iwoffii JCM 6840T A.junii DMST 2274T, Acinetobacter genospecies 3 DMST 2272T, A.haemolyticus DMST 2273T, A.johnsonii DMST 2276T, Acinetobacter genospecies 6 DMST 2275T, and A.radioresistens JCM 9326T. The strains tested contained Q-8 ro Q-9 as a major ubiquinone component. The identification of 52 isolates by Vitek 32 system showed that 23 isolates were A.baumannii, 18 isolates were A.calcoaceticus-A.baumannii complex (Abc complex) and 11 isolates were identified as A.Iwoffii I A.junnii. The identification of Acinetobacter species based on DNA relatedness method revealed to be more appropriate than the phenotypic method and Vitek 32 system

บรรณานุกรม :
Chutima Sereekul . (2544). Identification of acinetobacter species isolated from clinical specimen by photobiotin labelling DNA-DNA hybridization.
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Chutima Sereekul . 2544. "Identification of acinetobacter species isolated from clinical specimen by photobiotin labelling DNA-DNA hybridization".
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Chutima Sereekul . "Identification of acinetobacter species isolated from clinical specimen by photobiotin labelling DNA-DNA hybridization."
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2544. Print.
Chutima Sereekul . Identification of acinetobacter species isolated from clinical specimen by photobiotin labelling DNA-DNA hybridization. กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2544.