ชื่อเรื่อง | : | Bioactive compounds from endophytic fungi Phomopsis sp. from Urobotrya siamensis and isolate LRUB 20 from Leea rubra |
นักวิจัย | : | Porntep Chomcheon, 1977- |
คำค้น | : | Bioactive compounds , Urobotrya siamensis , Leea rubra , Endophytic fungi |
หน่วยงาน | : | จุฬาลงกรณ์มหาวิทยาลัย |
ผู้ร่วมงาน | : | Nattaya Ngamrojanavanich , Nongluksna Sriubolmas , Prasat Kittakoop , Chulalongkorn University. Faculty of Science |
ปีพิมพ์ | : | 2547 |
อ้างอิง | : | 9745315516 , http://cuir.car.chula.ac.th/handle/123456789/4096 |
ที่มา | : | - |
ความเชี่ยวชาญ | : | - |
ความสัมพันธ์ | : | - |
ขอบเขตของเนื้อหา | : | - |
บทคัดย่อ/คำอธิบาย | : | Thesis (M.Sc.)--Chulalongkorn University, 2004 The purpose of this research was to isolate bioactive compounds from endophytic fungi isolate LRUB 20 from Leea rubra Blume Ex Spreng. and isolate USIA 5 from Urobotrya siamensis Hiepko. Crude extract of endophytic fungus isolate LRUB 20 was purified by chromatographic techniques to afford three compounds, which were identified as asterric acid, 2-hydroxymethyl-3-methyl-cyclopent-2-enone, and 2-hydroxymethyl-3-methyl-cyclopentanone. The crude extract of endophytic fungus isolate USIA 5 provided 3-nitropropionic acid. The chemical structures of the isolated compounds were elucidated through extensive analyses of UV, IR, MS, and NMR and by comparison with literature. Asterric acid, 2-hydroxymethyl-3-methyl-cyclopent-2-enone, and 3-nitropropionic acid were found to exhibit activity against Mycobacterium tuberculosis H37Rv with the MIC values of 200, 200, and 0.39 g/ml, respectively. Based on morphology and nucleotide sequences of ITS1-5.8S-ITS2 regions of rDNA, endophytic fungus isolate USIA 5 was identified as Phomopsis sp. in the family Diaporthaceae. While based on morphology, the fungus isolate LRUB 20 limited in spore formation. Nucleotide sequences of ITS1-5.8S-ITS2 regions of rDNA were applied to classify endophytic fungus isolate LRUB 20, which was found to be in the family Magnaporthaceae |
บรรณานุกรม | : |
Porntep Chomcheon, 1977- . (2547). Bioactive compounds from endophytic fungi Phomopsis sp. from Urobotrya siamensis and isolate LRUB 20 from Leea rubra.
กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย. Porntep Chomcheon, 1977- . 2547. "Bioactive compounds from endophytic fungi Phomopsis sp. from Urobotrya siamensis and isolate LRUB 20 from Leea rubra".
กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย. Porntep Chomcheon, 1977- . "Bioactive compounds from endophytic fungi Phomopsis sp. from Urobotrya siamensis and isolate LRUB 20 from Leea rubra."
กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2547. Print. Porntep Chomcheon, 1977- . Bioactive compounds from endophytic fungi Phomopsis sp. from Urobotrya siamensis and isolate LRUB 20 from Leea rubra. กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2547.
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