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The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells
นักวิจัย : Xue, Cao , Kwek, Kenneth Y. C. , Chan, Jerry K. Y. , Chen, Qingfeng , Lim, Mayasari
คำค้น : DRNTU::Science::Biological sciences::Human anatomy and physiology
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Xue, C., Kwek, K. Y. C., Chan, J. K. Y., Chen, Q., & Lim, M. (2014). The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells. Biotechnology journal, 9(7), 980-989. , 1860-6768 , http://hdl.handle.net/10220/25034 , http://dx.doi.org/10.1002/biot.201300320
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biotechnology journal
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The bone marrow microenvironment plays an integral role in the regulation of hematopoiesis. Residing stromal cells and the extracellular matrix in the bone marrow microenvironment provide biological signals that control hematopoietic stem cell (HSC) function. In this study, we developed a bio-mimetic co-culture platform using the hollow fiber bioreactor (HFBR) for ex vivo expansion of HSCs. We evaluated the efficacy of such a platform in comparison to standard cultures performed on tissue culture polystyrene (TCP), using a human stromal cell line (HS-5) as stromal support, co-cultured with lineage-depleted human cord blood cells in serum-free medium supplemented with a cytokine cocktail. Our results showed that the performance of the HFBR in supporting total cell and CD34+ progenitor cell expansion was comparable to that of cultures on TCP. Cells harvested from the HFBR had a higher clonogenic ability. The performance of ex vivo-expanded cells from the HFBR in hematopoietic reconstitution in humanized mice was comparable to that of the TCP control. Scanning electron microscopy revealed that stroma cell growth inside the HFBR created a three-dimensional cell matrix architecture. These findings demonstrate the feasibility of utilizing the HFBR for creating a complex cell matrix architecture, which may provide good in vitro mimicry of the bone marrow, supporting large-scale expansion of HSCs.

บรรณานุกรม :
Xue, Cao , Kwek, Kenneth Y. C. , Chan, Jerry K. Y. , Chen, Qingfeng , Lim, Mayasari . (2557). The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Xue, Cao , Kwek, Kenneth Y. C. , Chan, Jerry K. Y. , Chen, Qingfeng , Lim, Mayasari . 2557. "The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Xue, Cao , Kwek, Kenneth Y. C. , Chan, Jerry K. Y. , Chen, Qingfeng , Lim, Mayasari . "The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Xue, Cao , Kwek, Kenneth Y. C. , Chan, Jerry K. Y. , Chen, Qingfeng , Lim, Mayasari . The hollow fiber bioreactor as a stroma-supported, serum-free ex vivo expansion platform for human umbilical cord blood cells. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.