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Expression, purification, crystallization and preliminary X-ray analysis of full-length human RIG-I

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Expression, purification, crystallization and preliminary X-ray analysis of full-length human RIG-I
นักวิจัย : Kwok, Jane , Hui, Kenrie P. Y. , Lescar, Julien , Kotaka, Masayo
คำค้น : DRNTU::Science::Biological sciences
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Kwok, J., Hui, K. P. Y., Lescar, J., & Kotaka, M. (2014). Expression, purification, crystallization and preliminary X-ray analysis of full-length human RIG-I. Acta crystallographica section F structural biology communications, 70(2), 248-251. , 2053-230X , http://hdl.handle.net/10220/25042 , http://dx.doi.org/10.1107/S2053230X14000430
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Acta crystallographica section F : structural biology communications
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The human innate immune system can detect invasion by microbial pathogens through pattern-recognition receptors that recognize structurally conserved pathogen-associated molecular patterns. Retinoic acid-inducible gene I (RIG-I)-like helicases (RLHs) are one of the two major families of pattern-recognition receptors that can detect viral RNA. RIG-I, belonging to the RLH family, is capable of recognizing intracellular viral RNA from RNA viruses, including influenza virus and Ebola virus. Here, full-length human RIG-I (hRIG-I) was cloned in Escherichia coli and expressed in a recombinant form with a His-SUMO tag. The protein was purified and crystallized at 291 K using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 2.85 Å resolution; the crystal belonged to space group F23, with unit-cell parameters a = b = c = 216.43 Å, [alpha] = [beta] = [gamma] = 90°.

บรรณานุกรม :
Kwok, Jane , Hui, Kenrie P. Y. , Lescar, Julien , Kotaka, Masayo . (2557). Expression, purification, crystallization and preliminary X-ray analysis of full-length human RIG-I.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Kwok, Jane , Hui, Kenrie P. Y. , Lescar, Julien , Kotaka, Masayo . 2557. "Expression, purification, crystallization and preliminary X-ray analysis of full-length human RIG-I".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Kwok, Jane , Hui, Kenrie P. Y. , Lescar, Julien , Kotaka, Masayo . "Expression, purification, crystallization and preliminary X-ray analysis of full-length human RIG-I."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Kwok, Jane , Hui, Kenrie P. Y. , Lescar, Julien , Kotaka, Masayo . Expression, purification, crystallization and preliminary X-ray analysis of full-length human RIG-I. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.