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Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip
นักวิจัย : Wu, Bo , Chen, Li-Chan , Huang, Youju , Zhang, Yiming , Kang, Yuejun , Kim, Dong-Hwan
คำค้น : DRNTU::Engineering::Chemical engineering::Biochemical engineering
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Wu, B., Chen, L. C., Huang, Y., Zhang, Y., Kang, Y., & Kim, D. H. (2014). Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip. Plasmonics, 9(4), 801-807. , http://hdl.handle.net/10220/24981 , http://dx.doi.org/10.1007/s11468-013-9661-4
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Plasmonics
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane (PDMS) layers and glass substrates with a precisely controlled thickness. The thin and flat microfluidic chip fitted the narrow space of dark-field microscopy and enabled the recording of single nanoparticle LSPR responses. Aptamer-antigen-antibody sandwiched detection scheme was utilized to enhance the LSPR responses for label-free biomolecular detection. This microfluidic chip successfully demonstrated the multiplexed detection of three independent analytes (PSA, IgE, and thrombin).

บรรณานุกรม :
Wu, Bo , Chen, Li-Chan , Huang, Youju , Zhang, Yiming , Kang, Yuejun , Kim, Dong-Hwan . (2557). Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wu, Bo , Chen, Li-Chan , Huang, Youju , Zhang, Yiming , Kang, Yuejun , Kim, Dong-Hwan . 2557. "Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wu, Bo , Chen, Li-Chan , Huang, Youju , Zhang, Yiming , Kang, Yuejun , Kim, Dong-Hwan . "Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Wu, Bo , Chen, Li-Chan , Huang, Youju , Zhang, Yiming , Kang, Yuejun , Kim, Dong-Hwan . Multiplexed biomolecular detection based on single nanoparticles immobilized on pneumatically controlled microfluidic chip. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.