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Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis
นักวิจัย : Hoo, Regina , Lam, Jian Hang , Huot, Ludovic , Pant, Aakanksha , Li, Rui , Hot, David , Alonso, Sylvie
คำค้น : DRNTU::Science::Biological sciences::Microbiology::Bacteria
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : Hozbor, Daniela Flavia
ปีพิมพ์ : 2557
อ้างอิง : Hoo, R., Lam, J. H., Huot, L., Pant, A., Li, R., Hot, D., et al. (2014). Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis. PLoS One, 9(12), e115243-. , 1932-6203 , http://hdl.handle.net/10220/24600 , http://dx.doi.org/10.1371/journal.pone.0115243
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS One
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Polysaccharide (PS) capsules are important virulence determinants for many bacterial pathogens. Bordetella pertussis, the agent of whooping cough, produces a surface associated microcapsule but its role in pertussis pathogenesis remained unknown. Here we showed that the B. pertussis capsule locus is expressed in vivo in murine lungs and that absence of the membrane-associated protein KpsT, involved in the transport of the PS polymers across the envelope, but not the surface-exposed PS capsule itself, affects drastically B. pertussis colonization efficacy in mice. Microarray analysis revealed that absence of KpsT in B. pertussis resulted in global down-regulation of gene expression including key virulence genes regulated by BvgA/S, the master two-component system. Using a BvgS phase-locked mutant, we demonstrated a functional link between KpsT and BvgA/S-mediated signal transduction. Whereas pull-down assays do not support physical interaction between BvgS sensor and any of the capsule locus encoded proteins, absence of KpsT impaired BvgS oligomerization, necessary for BvgS function. Furthermore, complementation studies indicated that instead of KpsT alone, the entire PS capsule transport machinery spanning the cell envelope likely plays a role in BvgS-mediated signal transduction. Our work thus provides the first experimental evidence of a role for a virulence-repressed gene in pertussis pathogenesis.

บรรณานุกรม :
Hoo, Regina , Lam, Jian Hang , Huot, Ludovic , Pant, Aakanksha , Li, Rui , Hot, David , Alonso, Sylvie . (2557). Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Hoo, Regina , Lam, Jian Hang , Huot, Ludovic , Pant, Aakanksha , Li, Rui , Hot, David , Alonso, Sylvie . 2557. "Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Hoo, Regina , Lam, Jian Hang , Huot, Ludovic , Pant, Aakanksha , Li, Rui , Hot, David , Alonso, Sylvie . "Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Hoo, Regina , Lam, Jian Hang , Huot, Ludovic , Pant, Aakanksha , Li, Rui , Hot, David , Alonso, Sylvie . Evidence for a role of the polysaccharide capsule transport proteins in pertussis pathogenesis. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.