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The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain
นักวิจัย : Rawling, David C. , Kohlway, Andrew S. , Luo, Dahai , Ding, Steve C. , Pyle, Anna Marie
คำค้น : DRNTU::Science::Medicine
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Rawling, D. C., Kohlway, A. S., Luo, D., Ding, S. C., & Pyle, A. M. (2014). The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain. Nucleic acids research, 42(18), 11601-11611. , 0305-1048 , http://hdl.handle.net/10220/24585 , http://dx.doi.org/10.1093/nar/gku817
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nucleic acids research
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Retinoic acid-inducible gene I (RIG-I) is a pattern recognition receptor expressed in metazoan cells that is responsible for eliciting the production of type I interferons and pro-inflammatory cytokines upon detection of intracellular, non-self RNA. Structural studies of RIG-I have identified a novel Pincer domain composed of two alpha helices that physically tethers the C-terminal domain to the SF2 helicase core. We find that the Pincer plays an important role in mediating the enzymatic and signaling activities of RIG-I. We identify a series of mutations that additively decouple the Pincer motif from the ATPase core and show that this decoupling results in impaired signaling. Through enzymological and biophysical analysis, we further show that the Pincer domain controls coupled enzymatic activity of the protein through allosteric control of the ATPase core. Further, we show that select regions of the HEL1 domain have evolved to potentiate interactions with the Pincer domain, resulting in an adapted ATPase cleft that is now responsive to adjacent domains that selectively bind viral RNA.

บรรณานุกรม :
Rawling, David C. , Kohlway, Andrew S. , Luo, Dahai , Ding, Steve C. , Pyle, Anna Marie . (2557). The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Rawling, David C. , Kohlway, Andrew S. , Luo, Dahai , Ding, Steve C. , Pyle, Anna Marie . 2557. "The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Rawling, David C. , Kohlway, Andrew S. , Luo, Dahai , Ding, Steve C. , Pyle, Anna Marie . "The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Rawling, David C. , Kohlway, Andrew S. , Luo, Dahai , Ding, Steve C. , Pyle, Anna Marie . The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.