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Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles
นักวิจัย : Ma, Xing , Devi, Gitali , Qu, Qiuyu , Toh, Desiree-Faye Kaixin , Chen, Gang , Zhao, Yanli
คำค้น : DRNTU::Science::Chemistry::Biochemistry
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Ma, X., Devi, G., Qu, Q., Toh, D.-F. K., Chen, G., & Zhao, Y. (2014). Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles. Bioconjugate chemistry, 25(8), 1412-1420. , http://hdl.handle.net/10220/24576 , http://dx.doi.org/10.1021/bc5002714
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Bioconjugate chemistry
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

In order to overcome poor cell permeability of antisense peptide nucleic acid (PNA), a fluorescent mesoporous silica nanoparticle (MSNP) carrier was developed to successfully deliver antisense PNA into cancer cells for effective silence of B-cell lymphoma 2 (Bcl-2) protein expression in vitro. First, fluorescent MSNP functionalized with disulfide bond bridged groups was fabricated and characterized. Antisense and negative control PNAs were synthesized and further conjugated with fluorescent dye cyanine 5. Then, the PNAs were covalently connected with fluorescent MSNP via amidation between amino group of PNAs and carboxylic acid group on the MSNP surface. High intracellular concentration of glutathione serves as a natural reducing agent, which could cleave the disulfide bond to trigger the PNA release in vitro. Confocal laser scanning microscopy studies prove that PNA conjugated MSNP was endocytosed by HeLa cancer cells, and redox-controlled intracellular release of antisense PNA from fluorescent MSNP was successfully achieved. Finally, effective silencing of the Bcl-2 protein expression induced by the delivered antisense PNA into HeLa cells was confirmed by Western blot assay.

NRF (Natl Research Foundation, S’pore)

ASTAR (Agency for Sci., Tech. and Research, S’pore)

MOE (Min. of Education, S’pore)

บรรณานุกรม :
Ma, Xing , Devi, Gitali , Qu, Qiuyu , Toh, Desiree-Faye Kaixin , Chen, Gang , Zhao, Yanli . (2557). Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ma, Xing , Devi, Gitali , Qu, Qiuyu , Toh, Desiree-Faye Kaixin , Chen, Gang , Zhao, Yanli . 2557. "Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ma, Xing , Devi, Gitali , Qu, Qiuyu , Toh, Desiree-Faye Kaixin , Chen, Gang , Zhao, Yanli . "Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Ma, Xing , Devi, Gitali , Qu, Qiuyu , Toh, Desiree-Faye Kaixin , Chen, Gang , Zhao, Yanli . Intracellular delivery of antisense peptide nucleic acid by fluorescent mesoporous silica nanoparticles. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.