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Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA
นักวิจัย : Wang, Zhijuan , Zhang, Juan , Zhu, Changfeng , Wu, Shixin , Mandler, Daniel , Marks, Robert S. , Zhang, Hua
คำค้น : DRNTU::Engineering::Materials::Biomaterials
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Wang, Z., Zhang, J., Zhu, C., Wu, S., Mandler, D., Marks, R. S., et al. (2014). Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA. Nanoscale, 6(6), 3110-3115. , 2040-3364 , http://hdl.handle.net/10220/24464 , http://dx.doi.org/10.1039/c3nr05625b
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nanoscale
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A sensitive electrochemical DNA biosensor based on the amplification of Au nanoparticles (AuNPs) has been developed. The AuNPs were modified with two types of signaling reporter DNAs, i.e. a methylene blue probe (MB-probe 2-SH) and T10 with a methylene blue signaling molecule (MB-T10-SH), forming DNA–AuNP conjugates. The MB-probe 2-SH is complementary to the target DNA, while MB-T10-SH is not. The presence of MB-T10-SH reduces the cross-reaction between target DNA and MB-probe 2-SH on the AuNPs, resulting in increased sensitivity of the biosensor. In our assay, the DNA sensor is fabricated by immobilizing a capture probe on the surface of the Au electrode, which then hybridizes with the corresponding target DNA, and further hybridizes with a DNA–Au conjugate. The signal of MB is measured by differential pulse voltammetry, while the DNA–Au conjugate enables the detection of target DNA in the linear range of 10−13 to 10−8 M with the detection limit as low as 50 fM.

บรรณานุกรม :
Wang, Zhijuan , Zhang, Juan , Zhu, Changfeng , Wu, Shixin , Mandler, Daniel , Marks, Robert S. , Zhang, Hua . (2557). Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Zhijuan , Zhang, Juan , Zhu, Changfeng , Wu, Shixin , Mandler, Daniel , Marks, Robert S. , Zhang, Hua . 2557. "Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Zhijuan , Zhang, Juan , Zhu, Changfeng , Wu, Shixin , Mandler, Daniel , Marks, Robert S. , Zhang, Hua . "Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Wang, Zhijuan , Zhang, Juan , Zhu, Changfeng , Wu, Shixin , Mandler, Daniel , Marks, Robert S. , Zhang, Hua . Amplified detection of femtomolar DNA based on a one-to-few recognition reaction between DNA-Au conjugate and target DNA. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.