ชื่อเรื่อง | : | Biomimetic production of silk-like recombinant squid sucker ring teeth proteins |
นักวิจัย | : | Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali |
คำค้น | : | DRNTU::Science::Biological sciences::Molecular biology |
หน่วยงาน | : | Nanyang Technological University, Singapore |
ผู้ร่วมงาน | : | - |
ปีพิมพ์ | : | 2557 |
อ้างอิง | : | Ding, D., Guerette, P. A., Hoon, S., Kong, K. W., Cornvik, T., Nilsson, M., et al. (2014). Biomimetic production of silk-like recombinant squid sucker ring teeth proteins. Biomacromolecules, 15(9), 3278-3289. , 1525-7797 , http://hdl.handle.net/10220/24460 , http://dx.doi.org/10.1021/bm500670r |
ที่มา | : | - |
ความเชี่ยวชาญ | : | - |
ความสัมพันธ์ | : | Biomacromolecules |
ขอบเขตของเนื้อหา | : | - |
บทคัดย่อ/คำอธิบาย | : | The sucker ring teeth (SRT) of Humboldt squid exhibit mechanical properties that rival those of robust engineered synthetic polymers. Remarkably, these properties are achieved without a mineral phase or covalent cross-links. Instead, SRT are exclusively made of silk-like proteins called “suckerins”, which assemble into nanoconfined β-sheet reinforced supramolecular networks. In this study, three streamlined strategies for full-length recombinant suckerin protein production and purification were developed. Recombinant suckerin exhibited high solubility and colloidal stability in aqueous-based solvents. In addition, the colloidal suspensions exhibited a concentration-dependent conformational switch, from random coil to β-sheet enriched structures. Our results demonstrate that recombinant suckerin can be produced in a facile manner in E. coli and processed from mild aqueous solutions into materials enriched in β-sheets. We suggest that recombinant suckerin-based materials offer potential for a range of biomedical and engineering applications. |
บรรณานุกรม | : |
Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali . (2557). Biomimetic production of silk-like recombinant squid sucker ring teeth proteins.
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali . 2557. "Biomimetic production of silk-like recombinant squid sucker ring teeth proteins".
กรุงเทพมหานคร : Nanyang Technological University, Singapore. Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali . "Biomimetic production of silk-like recombinant squid sucker ring teeth proteins."
กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print. Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali . Biomimetic production of silk-like recombinant squid sucker ring teeth proteins. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.
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