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Biomimetic production of silk-like recombinant squid sucker ring teeth proteins

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Biomimetic production of silk-like recombinant squid sucker ring teeth proteins
นักวิจัย : Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali
คำค้น : DRNTU::Science::Biological sciences::Molecular biology
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Ding, D., Guerette, P. A., Hoon, S., Kong, K. W., Cornvik, T., Nilsson, M., et al. (2014). Biomimetic production of silk-like recombinant squid sucker ring teeth proteins. Biomacromolecules, 15(9), 3278-3289. , 1525-7797 , http://hdl.handle.net/10220/24460 , http://dx.doi.org/10.1021/bm500670r
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Biomacromolecules
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The sucker ring teeth (SRT) of Humboldt squid exhibit mechanical properties that rival those of robust engineered synthetic polymers. Remarkably, these properties are achieved without a mineral phase or covalent cross-links. Instead, SRT are exclusively made of silk-like proteins called “suckerins”, which assemble into nanoconfined β-sheet reinforced supramolecular networks. In this study, three streamlined strategies for full-length recombinant suckerin protein production and purification were developed. Recombinant suckerin exhibited high solubility and colloidal stability in aqueous-based solvents. In addition, the colloidal suspensions exhibited a concentration-dependent conformational switch, from random coil to β-sheet enriched structures. Our results demonstrate that recombinant suckerin can be produced in a facile manner in E. coli and processed from mild aqueous solutions into materials enriched in β-sheets. We suggest that recombinant suckerin-based materials offer potential for a range of biomedical and engineering applications.

บรรณานุกรม :
Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali . (2557). Biomimetic production of silk-like recombinant squid sucker ring teeth proteins.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali . 2557. "Biomimetic production of silk-like recombinant squid sucker ring teeth proteins".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali . "Biomimetic production of silk-like recombinant squid sucker ring teeth proteins."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Ding, Dawei , Guerette, Paul A. , Hoon, Shawn , Kong, Kiat Whye , Cornvik, Tobias , Nilsson, Martina , Kumar, Akshita , Lescar, Julien , Miserez, Ali . Biomimetic production of silk-like recombinant squid sucker ring teeth proteins. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.