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Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS
นักวิจัย : Hao, Piliang , Ren, Yan , Pasterkamp, Gerard , Moll, Frans L. , de Kleijn, Dominique P. V. , Sze, Siu Kwan
คำค้น : DRNTU::Science::Biological sciences::Human anatomy and physiology
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Hao, P., Ren, Y., Pasterkamp, G., Moll, F. L., de Kleijn, D. P. V., & Sze, S. K. (2014). Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS. PROTEOMICS - clinical applications, 8(7-8), 631-635. , 1862-8346 , http://hdl.handle.net/10220/24147 , http://dx.doi.org/10.1002/prca.201400007
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PROTEOMICS - clinical applications
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Purpose: To increase the proteome coverage of human atherosclerotic plaques and identify low-abundance proteins that may have important functions during the development and progression of atherosclerosis. Experimental design: Thirty-eight human carotid atherosclerotic plaques were pooled into two samples and analyzed in triplicate using offline multidimensional LC-MS/MS. The collected fractions of trypsin-digested peptides from Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) were analyzed by LC-MS/MS on a Q Exactive (Thermo Fisher, MA, USA). Results: A total of 4702 proteins were identified from atherosclerotic plaques at a false discovery rate (FDR) of 1%, of which 3846 were identified with at least 2 unique peptides. Many pathways related to the development and progression of atherosclerosis were identified, such as atherosclerosis signaling, toll receptor signaling pathway and inhibition of matrix metalloproteases. Many low-abundance proteins with important functions in atherosclerosis that were previously unidentifiable using mass spectrometry based proteomics methods, such as TGF-β, interleukins and other growth factors, were identified confidently from plaques. Conclusions and clinical relevance: This study has substantially increased the coverage of the atherosclerotic plaque proteome which represents a leap forward in understanding of plaque composition, development and progression. The identification of many low-abundance proteins may also facilitate biomarker discovery.

บรรณานุกรม :
Hao, Piliang , Ren, Yan , Pasterkamp, Gerard , Moll, Frans L. , de Kleijn, Dominique P. V. , Sze, Siu Kwan . (2557). Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Hao, Piliang , Ren, Yan , Pasterkamp, Gerard , Moll, Frans L. , de Kleijn, Dominique P. V. , Sze, Siu Kwan . 2557. "Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Hao, Piliang , Ren, Yan , Pasterkamp, Gerard , Moll, Frans L. , de Kleijn, Dominique P. V. , Sze, Siu Kwan . "Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Hao, Piliang , Ren, Yan , Pasterkamp, Gerard , Moll, Frans L. , de Kleijn, Dominique P. V. , Sze, Siu Kwan . Deep proteomic profiling of human carotid atherosclerotic plaques using multidimensional LC-MS/MS. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.