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Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta
นักวิจัย : Patel, Jatin , Seppanen, Elke , Chong, Mark S. K. , Yeo, Julie S. L. , Teo, Erin Y. L. , Chan, Jerry K. Y. , Fisk, Nicholas M. , Khosrotehrani, Kiarash
คำค้น : DRNTU::Science::Chemistry::Biochemistry
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Patel, J., Seppanen, E., Chong, M. S. K., Yeo, J. S. L., Teo, E. Y. L., Chan, J. K. Y., et al. (2013). Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta. Stem cells translational medicine, 2(11), 839-847. , 2157-6564 , http://hdl.handle.net/10220/24036 , http://dx.doi.org/10.5966/sctm.2013-0092
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Stem cells translational medicine
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The term placenta is a highly vascularized tissue and is usually discarded upon birth. Our objective was to isolate clinically relevant quantities of fetal endothelial colony-forming cells (ECFCs) from human term placenta and to compare them to the well-established donor-matched umbilical cord blood (UCB)-derived ECFCs. A sorting strategy was devised to enrich for CD45−CD34+CD31Lo cells prior to primary plating to obtain pure placental ECFCs (PL-ECFCs) upon culture. UCB-ECFCs were derived using a well-described assay. PL-ECFCs were fetal in origin and expressed the same cell surface markers as UCB-ECFCs. Most importantly, a single term placenta could yield as many ECFCs as 27 UCB donors. PL-ECFCs and UCB-ECFCs had similar in vitro and in vivo vessel forming capacities and restored mouse hind limb ischemia in similar proportions. Gene expression profiles were only minimally divergent between PL-ECFCs and UCB-ECFCs, probably reflecting a vascular source versus a circulating source. Finally, PL-ECFCs and UCB-ECFCs displayed similar hierarchies between high and low proliferative colonies. We report a robust strategy to isolate ECFCs from human term placentas based on their cell surface expression. This yielded much larger quantities of ECFCs than UCB, but the cells were comparable in immunophenotype, gene expression, and in vivo functional ability. We conclude that PL-ECFCs have significant bio-banking and clinical translatability potential.

บรรณานุกรม :
Patel, Jatin , Seppanen, Elke , Chong, Mark S. K. , Yeo, Julie S. L. , Teo, Erin Y. L. , Chan, Jerry K. Y. , Fisk, Nicholas M. , Khosrotehrani, Kiarash . (2556). Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Patel, Jatin , Seppanen, Elke , Chong, Mark S. K. , Yeo, Julie S. L. , Teo, Erin Y. L. , Chan, Jerry K. Y. , Fisk, Nicholas M. , Khosrotehrani, Kiarash . 2556. "Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Patel, Jatin , Seppanen, Elke , Chong, Mark S. K. , Yeo, Julie S. L. , Teo, Erin Y. L. , Chan, Jerry K. Y. , Fisk, Nicholas M. , Khosrotehrani, Kiarash . "Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Patel, Jatin , Seppanen, Elke , Chong, Mark S. K. , Yeo, Julie S. L. , Teo, Erin Y. L. , Chan, Jerry K. Y. , Fisk, Nicholas M. , Khosrotehrani, Kiarash . Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.