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Design, synthesis, and application of electroactive reporters for nucleic acid biosensing.

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Design, synthesis, and application of electroactive reporters for nucleic acid biosensing.
นักวิจัย : Tansil, Natalia Chendrawati.
คำค้น : DRNTU::Engineering::Materials::Biomaterials. , DRNTU::Science::Chemistry::Analytical chemistry::Nucleic acids. , DRNTU::Science::Medicine::Biosensors.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2551
อ้างอิง : Tansil, N. C. (2008). Design, synthesis, and application of electroactive reporters for nucleic acid biosensing. Doctoral thesis, Nanyang Technological University, Singapore. , http://hdl.handle.net/10356/13541
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

This thesis discusses the design, synthesis, and application of electroactive reporters for nucleic acid biosensing. The reporters are able to specifically recognize and sensitively report the hybridization of target nucleic acids to capture probes in an electrochemical biosensor. Three compounds were used in different detection schemes. In each scheme, direct detection was achieved by measuring the electroactivity of Os or Ru complex. Lower detection limits were obtained through various amplification strategies. The Os complex catalytically oxidizes ascorbic acid, leading to an amplified current signal in amperometry and a detection limit of 60 fM for oligonucleotide samples. The Ru complex catalytically oxidizes guanine bases, leading to an increase of peak current in cyclic voltammetry and a detection limit of 1.5 pM for TP53 cDNA. The EDOT moiety acts as ‘seeds’ that facilitates selective polymerization of poly(EDOT). Under controlled conditions, the amount of polymer deposited was positively related to target DNA concentrations as low as 20 pM. The advantageous electrochemical and binding properties of the newly synthesized reporters qualify them for application in electrochemical detection platforms that can potentially be incorporated into point-of-care nucleic acid biosensors.

บรรณานุกรม :
Tansil, Natalia Chendrawati. . (2551). Design, synthesis, and application of electroactive reporters for nucleic acid biosensing..
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Tansil, Natalia Chendrawati. . 2551. "Design, synthesis, and application of electroactive reporters for nucleic acid biosensing.".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Tansil, Natalia Chendrawati. . "Design, synthesis, and application of electroactive reporters for nucleic acid biosensing.."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2551. Print.
Tansil, Natalia Chendrawati. . Design, synthesis, and application of electroactive reporters for nucleic acid biosensing.. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2551.