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Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications
นักวิจัย : Heidari, Amir , Yoon, Yong-Jin , Park, Woo-Tae , Su, Pei-Chen , Miao, Jianmin , Lin, Julius Tsai Ming , Park, Mi Kyoung
คำค้น : DRNTU::Engineering::Mechanical engineering::Bio-mechatronics
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Heidari, A., Yoon, Y.-J., Park, W.-T., Su, P.-C., Miao, J., Lin, J. T. M., et al. (2014). Biotin-Streptavidin Binding Interactions of Dielectric Filled Silicon Bulk Acoustic Resonators for Smart Label-Free Biochemical Sensor Applications. Sensors, 14(3), 4585-4598. , 1424-8220 , http://hdl.handle.net/10220/20369 , http://dx.doi.org/10.3390/s140304585
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Sensors
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Sensor performance of a dielectric filled silicon bulk acoustic resonator type label-free biosensor is verified with biotin-streptavidin binding interactions as a model system. The mass sensor is a micromachined silicon square plate with a dielectric filled capacitive excitation mechanism. The resonance frequency of the biotin modified resonator decreased 315 ppm when exposed to streptavidin solution for 15 min with a concentration of 10−7 M, corresponding to an added mass of 3.43 ng on the resonator surface. An additional control is added by exposing a bovine serum albumin (BSA)-covered device to streptavidin in the absence of the attached biotin. No resonance frequency shift was observed in the control experiment, which confirms the specificity of the detection. The sensor-to-sensor variability is also measured to be 4.3%. Consequently, the developed sensor can be used to observe in biotin-streptavidin interaction without the use of labelling or molecular tags. In addition, biosensor can be used in a variety of different immunoassay tests.

บรรณานุกรม :
Heidari, Amir , Yoon, Yong-Jin , Park, Woo-Tae , Su, Pei-Chen , Miao, Jianmin , Lin, Julius Tsai Ming , Park, Mi Kyoung . (2557). Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Heidari, Amir , Yoon, Yong-Jin , Park, Woo-Tae , Su, Pei-Chen , Miao, Jianmin , Lin, Julius Tsai Ming , Park, Mi Kyoung . 2557. "Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Heidari, Amir , Yoon, Yong-Jin , Park, Woo-Tae , Su, Pei-Chen , Miao, Jianmin , Lin, Julius Tsai Ming , Park, Mi Kyoung . "Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Heidari, Amir , Yoon, Yong-Jin , Park, Woo-Tae , Su, Pei-Chen , Miao, Jianmin , Lin, Julius Tsai Ming , Park, Mi Kyoung . Biotin-streptavidin binding interactions of dielectric filled silicon bulk acoustic resonators for smart label-free biochemical sensor applications. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.