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Diagnosing dengue at the point-of-care : utility of a rapid combined diagnostic kit in Singapore

หน่วยงาน Nanyang Technological University, Singapore

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ชื่อเรื่อง : Diagnosing dengue at the point-of-care : utility of a rapid combined diagnostic kit in Singapore
นักวิจัย : Gan, Victor C. , Tan, Li-Kiang , Lye, David C. , Pok, Kwoon-Yong , Mok, Shi-Qi , Chua, Rachel Choon-Rong , Leo, Yee-Sin , Ng, Lee-Ching
คำค้น : DRNTU::Science::Medicine
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : Bausch, Daniel G.
ปีพิมพ์ : 2557
อ้างอิง : Gan, V. C., Tan, L.-K., Lye, D. C., Pok, K.-Y., Mok, S.-Q., Chua, R. C.-R., et al. (2014). Diagnosing Dengue at the Point-of-Care: Utility of a Rapid Combined Diagnostic Kit in Singapore. PLoS ONE, 9(3), e90037-. , 1932-6203 , http://hdl.handle.net/10220/19516 , http://dx.doi.org/10.1371/journal.pone.0090037
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

WHO recommendations for dengue diagnosis require laboratory facilities. Antibody-based rapid diagnostic tests (RDTs) have performed poorly, and clinical diagnosis remains the mainstay in dengue-endemic countries. We evaluated a combination antigen-antibody RDT for point-of-care testing in a high-prevalence setting. In this prospective cohort study, adults were enrolled from a tertiary infectious disease centre for evaluation of undifferentiated febrile illness from October 2011 to May 2012. SD Bioline Dengue Duo was evaluated at point-of-care against a WHO-based reference standard of viral isolation, RT-PCR, NS1-, IgM-, and IgG-ELISA. 246 adults were enrolled (median age 34 years, range 18–69), of which 197 could be confirmed definitively as either dengue or non-dengue. DENV-2 was the predominant serotype (79.5%) and the ratio of primary to secondary cases was 1:1.1. There were no test failures and minimal interobserver variation with a Fleiss’ kappa of 0.983 (95% CI 0.827–1.00). Overall sensitivity and specificity were 93.9% (95% CI 88.8–96.8%) and 92.0% (95% CI 81.2–96.9%) respectively. Using WHO clinical criteria alone for diagnosis had similar sensitivities (95.9%, 95% CI 91.4–98.1%) and lower specificities (20.0%, 95% CI 11.2–33.0%). No significant difference in performance was found when testing early versus late presenters, primary versus secondary cases, or DENV-1 versus DENV-2 infections. The use of a combination RDT fulfills WHO ASSURED criteria for point-of-care testing and can enhance dengue diagnosis in an endemic setting. This has the potential to markedly improve clinical management of dengue in the field.

บรรณานุกรม :
Gan, Victor C. , Tan, Li-Kiang , Lye, David C. , Pok, Kwoon-Yong , Mok, Shi-Qi , Chua, Rachel Choon-Rong , Leo, Yee-Sin , Ng, Lee-Ching . (2557). Diagnosing dengue at the point-of-care : utility of a rapid combined diagnostic kit in Singapore.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Gan, Victor C. , Tan, Li-Kiang , Lye, David C. , Pok, Kwoon-Yong , Mok, Shi-Qi , Chua, Rachel Choon-Rong , Leo, Yee-Sin , Ng, Lee-Ching . 2557. "Diagnosing dengue at the point-of-care : utility of a rapid combined diagnostic kit in Singapore".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Gan, Victor C. , Tan, Li-Kiang , Lye, David C. , Pok, Kwoon-Yong , Mok, Shi-Qi , Chua, Rachel Choon-Rong , Leo, Yee-Sin , Ng, Lee-Ching . "Diagnosing dengue at the point-of-care : utility of a rapid combined diagnostic kit in Singapore."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Gan, Victor C. , Tan, Li-Kiang , Lye, David C. , Pok, Kwoon-Yong , Mok, Shi-Qi , Chua, Rachel Choon-Rong , Leo, Yee-Sin , Ng, Lee-Ching . Diagnosing dengue at the point-of-care : utility of a rapid combined diagnostic kit in Singapore. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.