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Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients

หน่วยงาน Nanyang Technological University, Singapore

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ชื่อเรื่อง : Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients
นักวิจัย : Kaur, Palvinder , Khong, Wei Xin , Wee, Sue Yuen , Tan, Eng Lee , Pipper, Juergen , Koay, Evelyn , Ng, Kah Ying , Yap, Joe Kwan , Chew, Kuan Kiat , Tan, Mei Ting , Leo, Yee Sin , Inoue, Masafumi , Ng, Oon Tek
คำค้น : DRNTU::Science::Medicine
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : Kumar, Anil
ปีพิมพ์ : 2557
อ้างอิง : Kaur, P., Khong, W. X., Wee, S. Y., Tan, E. L., Pipper, J., Koay, E., et al. (2014). Clinical Evaluation of a Low Cost, In-House Developed Real-Time RT-PCR Human Immunodeficiency Virus Type 1 (HIV-1) Quantitation Assay for HIV-1 Infected Patients. PLoS ONE, 9(3). , 1932-6203 , http://hdl.handle.net/10220/19482 , http://dx.doi.org/10.1371/journal.pone.0089826
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Objectives HIV-1 viral quantitation is essential for treatment monitoring. An in-house assay would decrease financial barriers to access. Materials and Methods A real-time competitive RT-PCR in house assay (Sing-IH) was developed in Singapore. Using HXB2 as reference, the assay's primers and probes were designed to generate a 183-bp product that overlaps a portion of the LTR region and gag region. A competitive internal control (IC) was included in each assay to monitor false negative results due to inhibition or human error. Clinical evaluation was performed on 249 HIV-1 positive patient samples in comparison with the commercially available Generic HIV Viral Load assay. Correlation and agreement of results were assessed for plasma HIV-1 quantification with both assays. Results The assay has a lower limit of detection equivalent to 126 copies/mL of HIV-1 RNA and a linear range of detection from 100–1000000 copies/mL. Comparative analysis with reference to the Generic assay demonstrated good agreement between both assays with a mean difference of 0.22 log10 copies/mL and 98.8% of values within 1 log10 copies/mL range. Furthermore, the Sing-IH assay can quantify HIV-1 group M subtypes A–H and group N isolates adequately, making it highly suitable for our region, where subtype B and CRF01_AE predominate. Conclusions With a significantly lower running cost compared to commercially available assays, the broadly sensitive Sing-IH assay could help to overcome the cost barriers and serve as a useful addition to the currently limited HIV viral load assay options for resource-limited settings.

บรรณานุกรม :
Kaur, Palvinder , Khong, Wei Xin , Wee, Sue Yuen , Tan, Eng Lee , Pipper, Juergen , Koay, Evelyn , Ng, Kah Ying , Yap, Joe Kwan , Chew, Kuan Kiat , Tan, Mei Ting , Leo, Yee Sin , Inoue, Masafumi , Ng, Oon Tek . (2557). Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Kaur, Palvinder , Khong, Wei Xin , Wee, Sue Yuen , Tan, Eng Lee , Pipper, Juergen , Koay, Evelyn , Ng, Kah Ying , Yap, Joe Kwan , Chew, Kuan Kiat , Tan, Mei Ting , Leo, Yee Sin , Inoue, Masafumi , Ng, Oon Tek . 2557. "Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Kaur, Palvinder , Khong, Wei Xin , Wee, Sue Yuen , Tan, Eng Lee , Pipper, Juergen , Koay, Evelyn , Ng, Kah Ying , Yap, Joe Kwan , Chew, Kuan Kiat , Tan, Mei Ting , Leo, Yee Sin , Inoue, Masafumi , Ng, Oon Tek . "Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Kaur, Palvinder , Khong, Wei Xin , Wee, Sue Yuen , Tan, Eng Lee , Pipper, Juergen , Koay, Evelyn , Ng, Kah Ying , Yap, Joe Kwan , Chew, Kuan Kiat , Tan, Mei Ting , Leo, Yee Sin , Inoue, Masafumi , Ng, Oon Tek . Clinical evaluation of a low cost, in-house developed real-time RT-PCR human immunodeficiency virus type 1 (HIV-1) quantitation assay for HIV-1 infected patients. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.