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Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments
นักวิจัย : Yildiz, Umit Hakan , De Hoog, Hans-Peter M. , Fu, Zhikang , Tomczak, Nikodem , Parikh, Atul N. , Nallani, Madhavan , Liedberg, Bo
คำค้น : DRNTU::Engineering::Materials
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Yildiz, U. H., De Hoog, H.-P. M., Fu, Z., Tomczak, N., Parikh, A. N., Nallani, M., et al. (2014). Third-Party ATP Sensing in Polymersomes: A Label-Free Assay of Enzyme Reactions in Vesicular Compartments. Small, 10(3), 442-447. , 1613-6810 , http://hdl.handle.net/10220/19139 , http://dx.doi.org/10.1002/smll.201300060
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Small
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Submicrometer, porous polymeric vesicles, composed of an amphiphilic di-block copolymer, polystyrene-b-polyisocyanoalanine (2-thiophene-3-yl-ethyl) amide, are used to encapsulate an enzyme, alkaline phosphatase, and a fluorescent reporter polymer poly 1(3((4methylthiophen-3-yl)oxy) propyl) quinuclidin-1-ium. Passive diffusion of exogenously added adenosine triphosphate (ATP) through the membrane was sensed by monitoring the ATP-induced fluorescence quenching of the reporter polymer followed by partial recovery of its emission due to hydrolysis of reporter-bound ATP by alkaline phosphatase.

บรรณานุกรม :
Yildiz, Umit Hakan , De Hoog, Hans-Peter M. , Fu, Zhikang , Tomczak, Nikodem , Parikh, Atul N. , Nallani, Madhavan , Liedberg, Bo . (2556). Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yildiz, Umit Hakan , De Hoog, Hans-Peter M. , Fu, Zhikang , Tomczak, Nikodem , Parikh, Atul N. , Nallani, Madhavan , Liedberg, Bo . 2556. "Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yildiz, Umit Hakan , De Hoog, Hans-Peter M. , Fu, Zhikang , Tomczak, Nikodem , Parikh, Atul N. , Nallani, Madhavan , Liedberg, Bo . "Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Yildiz, Umit Hakan , De Hoog, Hans-Peter M. , Fu, Zhikang , Tomczak, Nikodem , Parikh, Atul N. , Nallani, Madhavan , Liedberg, Bo . Third-party ATP sensing in polymersomes : a label-free assay of enzyme reactions in vesicular compartments. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.