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Synthetic multivalent antifungal peptides effective against fungi

หน่วยงาน Nanyang Technological University, Singapore

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ชื่อเรื่อง : Synthetic multivalent antifungal peptides effective against fungi
นักวิจัย : Lakshminarayanan, Rajamani , Liu, Shouping , Li, Jianguo , Nandhakumar, Muruganantham , Aung, Thet Tun , Goh, Eunice , Chang, Jamie Ya Ting , Saraswathi, Padhmanaban , Tang, Charles , Safie, Siti Radiah Binte , Lin, Lim Yih , Riezman, Howard , Lei, Zhou , Verma, Chandra S. , Beuerman, Roger W.
คำค้น : DRNTU::Science::Biological sciences::Biochemistry.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : Khan, Rizwan H.
ปีพิมพ์ : 2557
อ้างอิง : Lakshminarayanan, R., Liu, S., Li, J., Nandhakumar, M., Aung, T. T., Goh, E., et al. (2014). Synthetic multivalent antifungal peptides effective against fungi. PLoS ONE, 9(2), e87730-. , 1932-6203 , http://hdl.handle.net/10220/18996 , http://dx.doi.org/10.1371/journal.pone.0087730
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : PLoS ONE
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Taking advantage of the cluster effect observed in multivalent peptides, this work describes antifungal activity and possible mechanism of action of tetravalent peptide (B4010) which carries 4 copies of the sequence RGRKVVRR through a branched lysine core. B4010 displayed better antifungal properties than natamycin and amphotericin B. The peptide retained significant activity in the presence of monovalent/divalent cations, trypsin and serum and tear fluid. Moreover, B4010 is non-haemolytic and non-toxic to mice by intraperitoneal (200 mg/kg) or intravenous (100 mg/kg) routes. S. cerevisiae mutant strains with altered membrane sterol structures and composition showed hyper senstivity to B4010. The peptide had no affinity for cell wall polysaccharides and caused rapid dissipation of membrane potential and release of vital ions and ATP when treated with C. albicans. We demonstrate that additives which alter the membrane potential or membrane rigidity protect C. albicans from B4010-induced lethality. Calcein release assay and molecular dynamics simulations showed that the peptide preferentially binds to mixed bilayer containing ergosterol over phophotidylcholine-cholesterol bilayers. The studies further suggested that the first arginine is important for mediating peptide-bilayer interactions. Replacing the first arginine led to a 2–4 fold decrease in antifungal activities and reduced membrane disruption properties. The combined in silico and in vitro approach should facilitate rational design of new tetravalent antifungal peptides.

บรรณานุกรม :
Lakshminarayanan, Rajamani , Liu, Shouping , Li, Jianguo , Nandhakumar, Muruganantham , Aung, Thet Tun , Goh, Eunice , Chang, Jamie Ya Ting , Saraswathi, Padhmanaban , Tang, Charles , Safie, Siti Radiah Binte , Lin, Lim Yih , Riezman, Howard , Lei, Zhou , Verma, Chandra S. , Beuerman, Roger W. . (2557). Synthetic multivalent antifungal peptides effective against fungi.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Lakshminarayanan, Rajamani , Liu, Shouping , Li, Jianguo , Nandhakumar, Muruganantham , Aung, Thet Tun , Goh, Eunice , Chang, Jamie Ya Ting , Saraswathi, Padhmanaban , Tang, Charles , Safie, Siti Radiah Binte , Lin, Lim Yih , Riezman, Howard , Lei, Zhou , Verma, Chandra S. , Beuerman, Roger W. . 2557. "Synthetic multivalent antifungal peptides effective against fungi".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Lakshminarayanan, Rajamani , Liu, Shouping , Li, Jianguo , Nandhakumar, Muruganantham , Aung, Thet Tun , Goh, Eunice , Chang, Jamie Ya Ting , Saraswathi, Padhmanaban , Tang, Charles , Safie, Siti Radiah Binte , Lin, Lim Yih , Riezman, Howard , Lei, Zhou , Verma, Chandra S. , Beuerman, Roger W. . "Synthetic multivalent antifungal peptides effective against fungi."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Lakshminarayanan, Rajamani , Liu, Shouping , Li, Jianguo , Nandhakumar, Muruganantham , Aung, Thet Tun , Goh, Eunice , Chang, Jamie Ya Ting , Saraswathi, Padhmanaban , Tang, Charles , Safie, Siti Radiah Binte , Lin, Lim Yih , Riezman, Howard , Lei, Zhou , Verma, Chandra S. , Beuerman, Roger W. . Synthetic multivalent antifungal peptides effective against fungi. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.