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Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia
นักวิจัย : Datta, Arnab , Qian, Jingru , Chong, Ruifen , Kalaria, Raj N. , Francis, Paul , Lai, Mitchell K. P. , Chen, Christopher P. , Sze, Siu Kwan
คำค้น : DRNTU::Science::Biological sciences
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Datta, A., Qian, J., Chong, R., Kalaria, R. N., Francis, P., Lai, M. K. P., et. al. (2014) Novel Pathophysiological Markers are Revealed by iTRAQ-based Quantitative Clinical Proteomics Approach in Vascular Dementia. Journal of Proteomics. , http://hdl.handle.net/10220/18755 , 10.1016/j.jprot.2014.01.011 , 175806
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of proteomics
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Vascular dementia (VaD) is a leading cause of dementia in the elderly together with Alzheimer's disease with limited treatment options. Poor understanding of the pathophysiology underlying VaD is hindering the development of new therapies. Hence, to unravel its underlying molecular pathology, an iTRAQ-2D-LC–MS/MS strategy was used for quantitative analysis of pooled lysates from Brodmann area 21 of pathologically confirmed cases of VaD and matched non-neurological controls. A total of 144 differentially perturbed proteins out of 2284 confidently identified proteins (false discovery rate = 0.3%) were shortlisted for bioinformatics analysis. Western blot analysis of selected proteins using samples from individual patients (n = 10 per group) showed statistically significant increases in the abundance of SOD1 and NCAM and reduced ATP5A in VaD. This suggested a state of hypometabolism and vascular insufficiency along with an inflammatory condition during VaD. Elevation of SOD1 and increasing trend for iron-storage proteins (FTL, FTH1) may be indicative of an oxidative imbalance that is accompanied by an aberrant iron metabolism. The synaptic proteins did not exhibit a generalized decrease in abundance (e.g. syntaxin) in the VaD subjects. This reported proteome offers a reference data set for future basic or translational studies on VaD.

บรรณานุกรม :
Datta, Arnab , Qian, Jingru , Chong, Ruifen , Kalaria, Raj N. , Francis, Paul , Lai, Mitchell K. P. , Chen, Christopher P. , Sze, Siu Kwan . (2557). Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Datta, Arnab , Qian, Jingru , Chong, Ruifen , Kalaria, Raj N. , Francis, Paul , Lai, Mitchell K. P. , Chen, Christopher P. , Sze, Siu Kwan . 2557. "Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Datta, Arnab , Qian, Jingru , Chong, Ruifen , Kalaria, Raj N. , Francis, Paul , Lai, Mitchell K. P. , Chen, Christopher P. , Sze, Siu Kwan . "Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2557. Print.
Datta, Arnab , Qian, Jingru , Chong, Ruifen , Kalaria, Raj N. , Francis, Paul , Lai, Mitchell K. P. , Chen, Christopher P. , Sze, Siu Kwan . Novel pathophysiological markers are revealed by iTRAQ-based quantitative clinical proteomics approach in vascular dementia. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2557.