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Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus
นักวิจัย : Deng, Jiajia , Toh, Chee-Seng
คำค้น : DRNTU::Science::Medicine::Biosensors.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Deng, J., & Toh, C.-S. (2013). Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus. Sensors, 13(6), 7774-7785. , 1424-8220 , http://hdl.handle.net/10220/18740 , http://dx.doi.org/10.3390/s130607774
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Sensors
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A novel and integrated membrane sensing platform for DNA detection is developed based on an anodic aluminum oxide (AAO) membrane. Platinum electrodes (~50–100 nm thick) are coated directly on both sides of the alumina membrane to eliminate the solution resistance outside the nanopores. The electrochemical impedance technique is employed to monitor the impedance changes within the nanopores upon DNA binding. Pore resistance (Rp) linearly increases in response towards the increasing concentration of the target DNA in the range of 1 × 10−12 to 1 × 10−6 M. Moreover, the biosensor selectively differentiates the complementary sequence from single base mismatched (MM-1) strands and non-complementary strands. This study reveals a simple, selective and sensitive method to fabricate a label-free DNA biosensor.

บรรณานุกรม :
Deng, Jiajia , Toh, Chee-Seng . (2556). Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Deng, Jiajia , Toh, Chee-Seng . 2556. "Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Deng, Jiajia , Toh, Chee-Seng . "Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Deng, Jiajia , Toh, Chee-Seng . Impedimetric DNA biosensor based on a nanoporous alumina membrane for the detection of the specific oligonucleotide sequence of dengue virus. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.