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β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films
นักวิจัย : Low, Yuen Kei Adarina , Zou, Xi , Fang, Yuming , Wang, Junling , Lin, Weisi , Boey, Freddy Yin Chiang , Ng, Kee Woei
คำค้น : DRNTU::Engineering::Materials
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Low, Y. K. A., Zou, X., Fang, Y., Wang, J., Lin, W., Boey, F., et al. (2014). β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films. Materials science and engineering : C, 34(1), 345-353. , 0928-4931 , http://hdl.handle.net/10220/18751 , http://dx.doi.org/10.1016/j.msec.2013.09.029
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Materials science and engineering : C
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The piezoelectric response from β-phase poly (vinylidene fluoride) (PVDF) can potentially be exploited for biomedical application. We hypothesized that α and β-phase PVDF exert direct but different influence on cellular behavior. α- and β-phase PVDF films were synthesized through solution casting and characterized with FT-IR, XRD, AFM and PFM to ensure successful fabrication of α and β-phase PVDF films. Cellular evaluation with L929 mouse fibroblasts over one-week was conducted with AlamarBlue® metabolic assay and PicoGreen® proliferation assay. Immunostaining of fibronectin investigated the extent and distribution of extracellular matrix deposition. Image saliency analysis quantified differences in cellular distribution on the PVDF films. Our results showed that β-phase PVDF films with the largest area expressing piezoelectric effect elicited highest cell metabolic activity at day 3 of culture. Increased fibronectin adsorption towards the cell-material interface was shown on β-phase PVDF films. Image saliency analysis showed that fibroblasts on β-phase PVDF films were more homogeneously distributed than on α-phase PVDF films. Taken collectively, the different molecular packing of α and β-phase PVDF resulted in differing physical properties of films, which in turn induced differences in cellular behaviors. Further analysis of how α and β-phase PVDF may evoke specific cellular behavior to suit particular application will be intriguing.

บรรณานุกรม :
Low, Yuen Kei Adarina , Zou, Xi , Fang, Yuming , Wang, Junling , Lin, Weisi , Boey, Freddy Yin Chiang , Ng, Kee Woei . (2556). β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Low, Yuen Kei Adarina , Zou, Xi , Fang, Yuming , Wang, Junling , Lin, Weisi , Boey, Freddy Yin Chiang , Ng, Kee Woei . 2556. "β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Low, Yuen Kei Adarina , Zou, Xi , Fang, Yuming , Wang, Junling , Lin, Weisi , Boey, Freddy Yin Chiang , Ng, Kee Woei . "β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Low, Yuen Kei Adarina , Zou, Xi , Fang, Yuming , Wang, Junling , Lin, Weisi , Boey, Freddy Yin Chiang , Ng, Kee Woei . β-Phase poly(vinylidene fluoride) films encouraged more homogeneous cell distribution and more significant deposition of fibronectin towards the cell–material interface compared to α-phase poly(vinylidene fluoride) films. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.