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Biomimetic membrane platform : fabrication, characterization and applications

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Biomimetic membrane platform : fabrication, characterization and applications
นักวิจัย : Yildiz, Ahu Arslan , Yildiz, Umit Hakan , Bo, Liedberg , Sinner, Eva-Kathrin
คำค้น : DRNTU::Engineering::Materials::Biomaterials
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Yildiz, A. A., Yildiz, U. H., Liedberg, B., & Sinner, E. K. (2012). Biomimetic membrane platform : fabrication, characterization and applications. Colloids and surfaces B : biointerfaces, 103, 510-516. , 0927-7765 , http://hdl.handle.net/10220/17412 , http://dx.doi.org/10.1016/j.colsurfb.2012.10.066
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Colloids and surfaces B : biointerfaces
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A facile method for assembly of biomimetic membranes serving as a platform for expression and insertion of membrane proteins is described. The membrane architecture was constructed in three steps: (i) assembly/printing of α-laminin peptide (P19) spacer on gold to separate solid support from the membrane architecture; (ii) covalent coupling of different lipid anchors to the P19 layer to serve as stabilizers of the inner leaflet during bilayer formation; (iii) lipid vesicle spreading to form a complete bilayer. Two different lipid membrane systems were examined and two different P19 architectures prepared by either self-assembly or μ-contact printing were tested and characterized using contact angle (CA) goniometry, surface plasmon resonance (SPR) spectroscopy and imaging surface plasmon resonance (iSPR). It is shown that surface coverage of cushion layer is significantly improved by μ-contact printing thereby facilitating bilayer formation as compared to self-assembly. To validate applicability of proposed methodology, incorporation of Cytochrome bo3 ubiquinol oxidase (Cyt-bo3) into biomimetic membrane was performed by in vitro expression technique which was further monitored by surface plasmon enhanced fluorescence spectroscopy (SPFS). The results showed that solid supported planar membranes, tethered by α-laminin peptide cushion layer, provide an attractive environment for membrane protein insertion and characterization.

บรรณานุกรม :
Yildiz, Ahu Arslan , Yildiz, Umit Hakan , Bo, Liedberg , Sinner, Eva-Kathrin . (2555). Biomimetic membrane platform : fabrication, characterization and applications.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yildiz, Ahu Arslan , Yildiz, Umit Hakan , Bo, Liedberg , Sinner, Eva-Kathrin . 2555. "Biomimetic membrane platform : fabrication, characterization and applications".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yildiz, Ahu Arslan , Yildiz, Umit Hakan , Bo, Liedberg , Sinner, Eva-Kathrin . "Biomimetic membrane platform : fabrication, characterization and applications."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Yildiz, Ahu Arslan , Yildiz, Umit Hakan , Bo, Liedberg , Sinner, Eva-Kathrin . Biomimetic membrane platform : fabrication, characterization and applications. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.