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Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties
นักวิจัย : Li, Xiang , Li, Peng , Saravanan, Rathi , Basu, Anindya , Mishra, Biswajit , Lim, Suo Hon , Su, Xiaodi , Tambyah, Paul Anantharajah , Leong, Susanna Su Jan
คำค้น : DRNTU::Engineering::Materials::Biomaterials
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Li, X., Li, P., Saravanan, R., Basu, A., Mishra, B., Lim, S. H., et al. (2013). Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties. Acta biomaterialia, in press. , 1742-7061 , http://hdl.handle.net/10220/17670 , http://dx.doi.org/10.1016/j.actbio.2013.09.009
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Acta biomaterialia
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Catheter-associated urinary tract infections (CAUTIs) are often preceded by pathogen colonization on catheter surfaces and are a major health threat facing hospitals worldwide. Antimicrobial peptides (AMPs) are a class of new antibiotics that hold promise in curbing CAUTIs caused by antibiotic-resistant pathogens. This study aims to systematically evaluate the feasibility of immobilizing two newly engineered arginine/lysine/tryptophan-rich AMPs with broad antimicrobial spectra and salt-tolerant properties on silicone surfaces to address CAUTIs. The peptides were successfully immobilized on polydimethylsiloxane and urinary catheter surfaces via an allyl glycidyl ether (AGE) polymer brush interlayer, as confirmed by X-ray photoelectron spectroscopy and water contact angle analyses. The peptide-coated silicone surfaces exhibited excellent microbial killing activity towards bacteria and fungi in urine and in phosphate-buffered saline. Although both the soluble and immobilized peptides demonstrated membrane disruption capabilities, the latter showed a slower rate of kill, presumably due to reduced diffusivity and flexibility resulting from conjugation to the polymer brush. The synergistic effects of the AGE polymer brush and AMPs prevented biofilm formation by repelling cell adhesion. The peptide-coated surface showed no toxicity towards smooth muscle cells. The findings of this study clearly indicate the potential for the development of AMP-based coating platforms to prevent CAUTIs.

บรรณานุกรม :
Li, Xiang , Li, Peng , Saravanan, Rathi , Basu, Anindya , Mishra, Biswajit , Lim, Suo Hon , Su, Xiaodi , Tambyah, Paul Anantharajah , Leong, Susanna Su Jan . (2556). Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Li, Xiang , Li, Peng , Saravanan, Rathi , Basu, Anindya , Mishra, Biswajit , Lim, Suo Hon , Su, Xiaodi , Tambyah, Paul Anantharajah , Leong, Susanna Su Jan . 2556. "Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Li, Xiang , Li, Peng , Saravanan, Rathi , Basu, Anindya , Mishra, Biswajit , Lim, Suo Hon , Su, Xiaodi , Tambyah, Paul Anantharajah , Leong, Susanna Su Jan . "Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Li, Xiang , Li, Peng , Saravanan, Rathi , Basu, Anindya , Mishra, Biswajit , Lim, Suo Hon , Su, Xiaodi , Tambyah, Paul Anantharajah , Leong, Susanna Su Jan . Antimicrobial functionalization of silicone surfaces with engineered short peptides having broad spectrum antimicrobial and salt-resistant properties. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.