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Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE
นักวิจัย : Yu, Yong , Li, Jie , Chen, Sijie , Hong, Yuning , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Yu, Y., Li, J., Chen, S., Hong, Y., Ng, K. M., Luo, K. Q., et al. (2013). Thiol-Reactive Molecule with Dual-Emission-Enhancement Property for Specific Prestaining of Cysteine Containing Proteins in SDS-PAGE. ACS Applied Materials & Interfaces, 5(11), 4613-4616. , http://hdl.handle.net/10220/17286 , http://dx.doi.org/10.1021/am401486h
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : ACS applied materials & interfaces
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

1-[4-(Bromomethyl)phenyl]-1,2,2-triphenylethene (2) was synthesized and evaluated for specific fluorescent prestaining of proteins containing cysteine (Cys) in SDS-PAGE. The molecule showed classic aggregation-induced emission (AIE) property in protein labeling and its quantum efficiency was further enhanced upon reacting with Cys. The parameters of reaction such as labeling time and concentration of dye and reducing reagent-tris(2-carboxyethyl)phosphine (TCEP) were examined to obtain the optimal labeling condition. In addition to its specific labeling effect, molecule 2 also showed its advantage over traditional self-quenching dyes through labeling Cys containing BSA with different dye/Cys ratios.

บรรณานุกรม :
Yu, Yong , Li, Jie , Chen, Sijie , Hong, Yuning , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong . (2556). Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yu, Yong , Li, Jie , Chen, Sijie , Hong, Yuning , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong . 2556. "Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Yu, Yong , Li, Jie , Chen, Sijie , Hong, Yuning , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong . "Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Yu, Yong , Li, Jie , Chen, Sijie , Hong, Yuning , Ng, Ka Ming , Luo, Kathy Qian , Tang, Ben Zhong . Thiol-reactive molecule with dual-emission-enhancement property for specific prestaining of cysteine containing proteins in SDS-PAGE. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.