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Cell viability and angiogenic potential of a bioartificial adipose substitute

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Cell viability and angiogenic potential of a bioartificial adipose substitute
นักวิจัย : Panneerselvan, Anitha , Nguyen, Luong T. H. , Su, Yan , Teo, Wee Eong , Liao, Susan , Ramakrishna, Seeram , Chan, Ching Wan
คำค้น : DRNTU::Engineering::Materials::Biomaterials.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Panneerselvan, A., Nguyen, L. T. H., Su, Y., Teo, W. E., Liao, S., Ramakrishna, S., et al. (2012). Cell viability and angiogenic potential of a bioartificial adipose substitute. Journal of tissue engineering and regenerative medicine, in press. , 1932-6254 , http://hdl.handle.net/10220/17311 , http://dx.doi.org/10.1002/term.1633
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of tissue engineering and regenerative medicine
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

An implantable scaffold pre-seeded with cells needs to remain viable and encourage rapid angiogenesis in order to replace injured tissues, especially for tissue defect repairs. We created a bioartificial adipose graft composed of an electrospun 3D nanofibrous scaffold and fat tissue excised from New Zealand white rabbits. Cell viability and angiogenesis potential of the bioartificial substitute were examined during four weeks of culture in Dulbecco's Modified Eagle Medium by immunohistochemical staining with LIVE/DEAD® cell kit and PECAM-1 antibody, respectively. In addition, a Matrigel® assay was performed to examine the possibility of blood vessels sprouting from the bioartificial graft. Our results showed that cells within the graft were viable and vascular tubes were present at week 4, while cells in a fat tissue block were dead in vitro. In addition, capillaries were observed sprouting from the graft into the Matrigel, demonstrating its angiogenic potential. We expect that improved cell viability and angiogenesis in the bioartificial substitute, compared to intact autologous graft, could potentially contribute to its survival following implantation.

บรรณานุกรม :
Panneerselvan, Anitha , Nguyen, Luong T. H. , Su, Yan , Teo, Wee Eong , Liao, Susan , Ramakrishna, Seeram , Chan, Ching Wan . (2555). Cell viability and angiogenic potential of a bioartificial adipose substitute.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Panneerselvan, Anitha , Nguyen, Luong T. H. , Su, Yan , Teo, Wee Eong , Liao, Susan , Ramakrishna, Seeram , Chan, Ching Wan . 2555. "Cell viability and angiogenic potential of a bioartificial adipose substitute".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Panneerselvan, Anitha , Nguyen, Luong T. H. , Su, Yan , Teo, Wee Eong , Liao, Susan , Ramakrishna, Seeram , Chan, Ching Wan . "Cell viability and angiogenic potential of a bioartificial adipose substitute."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Panneerselvan, Anitha , Nguyen, Luong T. H. , Su, Yan , Teo, Wee Eong , Liao, Susan , Ramakrishna, Seeram , Chan, Ching Wan . Cell viability and angiogenic potential of a bioartificial adipose substitute. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.