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Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects
นักวิจัย : Schantz, Jan-Thorsten , Woodruff, Maria Ann , Lam, Chris X. F. , Lim, Thiam Chye , Machens, Hans Gunther , Teoh, Swee-Hin , Hutmacher, Dietmar Werner
คำค้น : DRNTU::Science::Medicine::Biomedical engineering.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Schantz, J.-T., Woodruff, M. A., Lam, C. X.F., Lim, T. C., Machens, H. G., Teoh, S.-H., et al. (2012). Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects. Journal of the mechanical behavior of biomedical baterials, 11, 132-142. , 1751-6161 , http://hdl.handle.net/10220/17186 , http://dx.doi.org/10.1016/j.jmbbm.2012.02.008
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of the mechanical behavior of biomedical baterials
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The complexity of stem cell lineage commitment requires studies to investigate the intrinsic and extrinsic regulatory events during differentiation. The objective of this long-term in vivo study was to investigate cellular differentiation and tissue formation of transplanted undifferentiated bone-marrow-derived mesenchymal progenitor cells (BMPCs) in combination with a medical grade polycaprolactone (mPCL) scaffold and to compare them to osteoblasts; a more differentiated cell type in a calvarial defect model. Tissue formation was assessed via histology, mechanical and radiological methods after 3 12, and 24 months. After 3 months our results indicated that transplanted mesenchymal progenitor cells were influenced by the niche of the host environment. Scaffold/BMPCs formed islands of bone tissue inside the defect area. However when the surrounding host calvarium contained a high content of fatty tissue, the fat content in the defect areas was also significantly higher. In contrast, defects repaired with scaffold/cOBs did not show this phenomenon. Analysis after 12 and 24 months confirmed these observations indicating that a predominantly fatty environment leads to adipogenic development in the progenitor group. Biomechanical data revealed that the tissue was less firm in the BMPC group compared to the cOB seeded group. Evaluation of cell plasticity in vivo has important consequences in clinical cell transplantation protocols. This study indicates that cell fate decisions are partially regulated by extrinsic control mechanisms of the immediate environment suggesting that induction of BMPCs into a specific lineage could be beneficial prior transplantation.

บรรณานุกรม :
Schantz, Jan-Thorsten , Woodruff, Maria Ann , Lam, Chris X. F. , Lim, Thiam Chye , Machens, Hans Gunther , Teoh, Swee-Hin , Hutmacher, Dietmar Werner . (2555). Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Schantz, Jan-Thorsten , Woodruff, Maria Ann , Lam, Chris X. F. , Lim, Thiam Chye , Machens, Hans Gunther , Teoh, Swee-Hin , Hutmacher, Dietmar Werner . 2555. "Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Schantz, Jan-Thorsten , Woodruff, Maria Ann , Lam, Chris X. F. , Lim, Thiam Chye , Machens, Hans Gunther , Teoh, Swee-Hin , Hutmacher, Dietmar Werner . "Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Schantz, Jan-Thorsten , Woodruff, Maria Ann , Lam, Chris X. F. , Lim, Thiam Chye , Machens, Hans Gunther , Teoh, Swee-Hin , Hutmacher, Dietmar Werner . Differentiation potential of mesenchymal progenitor cells following transplantation into calvarial defects. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.