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A unique Oct4 interface is crucial for reprogramming to pluripotency

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : A unique Oct4 interface is crucial for reprogramming to pluripotency
นักวิจัย : Esch, Daniel , Vahokoski, Juha , Groves, Matthew R. , Pogenberg, Vivian , Cojocaru, Vlad , vom Bruch, Hermann , Han, Dong , Drexler, Hannes C. A. , Araúzo-Bravo, Marcos J. , Ng, Calista K. L. , Jauch, Ralf , Wilmanns, Matthias , Schöler, Hans R.
คำค้น : DRNTU::Science::Biological sciences::Cytology
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Esch, D., Vahokoski, J., Groves, M. R., Pogenberg, V., Cojocaru, V., vom Bruch, H. V., et al. (2013). A unique Oct4 interface is crucial for reprogramming to pluripotency. Nature cell biology, 15(3), 295-301. , http://hdl.handle.net/10220/18028 , http://dx.doi.org/10.1038/ncb2680
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Nature cell biology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Terminally differentiated cells can be reprogrammed to pluripotency by the forced expression of Oct4, Sox2, Klf4 and c-Myc1, 2. However, it remains unknown how this leads to the multitude of epigenetic changes observed during the reprogramming process. Interestingly, Oct4 is the only factor that cannot be replaced by other members of the same family to induce pluripotency3, 4, 5. To understand the unique role of Oct4 in reprogramming, we determined the structure of its POU domain bound to DNA. We show that the linker between the two DNA-binding domains is structured as an α-helix and exposed to the protein’s surface, in contrast to the unstructured linker of Oct1. Point mutations in this α-helix alter or abolish the reprogramming activity of Oct4, but do not affect its other fundamental properties. On the basis of mass spectrometry studies of the interactome of wild-type and mutant Oct4, we propose that the linker functions as a protein–protein interaction interface and plays a crucial role during reprogramming by recruiting key epigenetic players to Oct4 target genes. Thus, we provide molecular insights to explain how Oct4 contributes to the reprogramming process.

บรรณานุกรม :
Esch, Daniel , Vahokoski, Juha , Groves, Matthew R. , Pogenberg, Vivian , Cojocaru, Vlad , vom Bruch, Hermann , Han, Dong , Drexler, Hannes C. A. , Araúzo-Bravo, Marcos J. , Ng, Calista K. L. , Jauch, Ralf , Wilmanns, Matthias , Schöler, Hans R. . (2556). A unique Oct4 interface is crucial for reprogramming to pluripotency.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Esch, Daniel , Vahokoski, Juha , Groves, Matthew R. , Pogenberg, Vivian , Cojocaru, Vlad , vom Bruch, Hermann , Han, Dong , Drexler, Hannes C. A. , Araúzo-Bravo, Marcos J. , Ng, Calista K. L. , Jauch, Ralf , Wilmanns, Matthias , Schöler, Hans R. . 2556. "A unique Oct4 interface is crucial for reprogramming to pluripotency".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Esch, Daniel , Vahokoski, Juha , Groves, Matthew R. , Pogenberg, Vivian , Cojocaru, Vlad , vom Bruch, Hermann , Han, Dong , Drexler, Hannes C. A. , Araúzo-Bravo, Marcos J. , Ng, Calista K. L. , Jauch, Ralf , Wilmanns, Matthias , Schöler, Hans R. . "A unique Oct4 interface is crucial for reprogramming to pluripotency."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Esch, Daniel , Vahokoski, Juha , Groves, Matthew R. , Pogenberg, Vivian , Cojocaru, Vlad , vom Bruch, Hermann , Han, Dong , Drexler, Hannes C. A. , Araúzo-Bravo, Marcos J. , Ng, Calista K. L. , Jauch, Ralf , Wilmanns, Matthias , Schöler, Hans R. . A unique Oct4 interface is crucial for reprogramming to pluripotency. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.