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A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system
นักวิจัย : Leong, Wenyan , Lau, Ting Ting , Wang, Dong-An
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Leong, W., Lau, T. T., & Wang, D.-A. (2013). A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system. Acta Biomaterialia, 9(5), 6459-6467. , 1742-7061 , http://hdl.handle.net/10220/17847 , http://dx.doi.org/10.1016/j.actbio.2012.10.047
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Acta biomaterialia
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

In this study, a novel therapeutic cell delivery methodology in the form of hydrogel encapsulating cell-laden microspheres was developed and investigated. As a model cell for cartilage tissue engineering, chondrocytes were successfully encapsulated in gelatin-based microspheres (mostly of diameter 50–100 μm, centred at 75–100 μm) with high cell viability during the formation of microspheres via a water-in-oil single emulsion process under a low temperature without any chemical treatment. These cell-laden microspheres were then encapsulated in alginate-based hydrogel constructs. By elevating the temperature to 37 °C, the cell-laden microspheres were completely dissolved within 2 days, resulting in the same number of same-sized spherical cavities in hydrogel bulk, along with which the encapsulated cells were released from the microspheres and suspended inside the cavities to be cultivated for further development. In this cell delivery system, the microspheres played a dual role as both removable cell vehicles and porogens for creation of the intra-hydrogel cavities, in which the delivered cells were provided with both free living spaces and a better permeable environment. This temperature-cured dissolvable gelatin microsphere-based cell carrier (tDGMC) associating with cell-laden hydrogel scaffold was attempted and evaluated through WST-1, quantitative polymerase chain reaction, biochemical assays and various immunohistochemistry and histology stains. The results indicate that tDGMC technology can facilitate the delivery of chondrocytes, as a non-anchorage-dependent therapeutic cell, with significantly greater efficiency.

บรรณานุกรม :
Leong, Wenyan , Lau, Ting Ting , Wang, Dong-An . (2555). A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Leong, Wenyan , Lau, Ting Ting , Wang, Dong-An . 2555. "A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Leong, Wenyan , Lau, Ting Ting , Wang, Dong-An . "A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Leong, Wenyan , Lau, Ting Ting , Wang, Dong-An . A temperature-cured dissolvable gelatin microsphere-based cell carrier for chondrocyte delivery in a hydrogel scaffolding system. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.