ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface
นักวิจัย : Zou, Changji , Larisika, Melanie , Nagy, Gabor , Srajer, Johannes , Oostenbrink, Chris , Chen, Xiaodong , Knoll, Wolfgang , Bo, Liedberg , Nowak, Christoph
คำค้น : DRNTU::Engineering::Materials
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Zou, C., Larisika, M., Nagy, G., Srajer, J., Oostenbrink, C., Chen, X., et al. (2013). Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced raman and infrared absorption spectroscopies on a two-layer gold surface. The journal of physical chemistry B, 117(33), 9606-9614. , http://hdl.handle.net/10220/17762 , http://dx.doi.org/10.1021/jp404573q
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : The journal of physical chemistry B
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The heme protein cytochrome c adsorbed to a two-layer gold surface modified with a self-assembled monolayer of 2-mercaptoethanol was analyzed using a two-dimensional (2D) heterospectral correlation analysis that combined surface-enhanced infrared absorption spectroscopy (SEIRAS) and surface-enhanced Raman spectroscopy (SERS). Stepwise increasing electric potentials were applied to alter the redox state of the protein and to induce conformational changes within the protein backbone. We demonstrate herein that 2D heterospectral correlation analysis is a particularly suitable and useful technique for the study of heme-containing proteins as the two spectroscopies address different portions of the protein. Thus, by correlating SERS and SEIRAS data in a 2D plot, we can obtain a deeper understanding of the conformational changes occurring at the redox center and in the supporting protein backbone during the electron transfer process. The correlation analyses are complemented by molecular dynamics calculations to explore the intramolecular interactions.

บรรณานุกรม :
Zou, Changji , Larisika, Melanie , Nagy, Gabor , Srajer, Johannes , Oostenbrink, Chris , Chen, Xiaodong , Knoll, Wolfgang , Bo, Liedberg , Nowak, Christoph . (2556). Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zou, Changji , Larisika, Melanie , Nagy, Gabor , Srajer, Johannes , Oostenbrink, Chris , Chen, Xiaodong , Knoll, Wolfgang , Bo, Liedberg , Nowak, Christoph . 2556. "Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zou, Changji , Larisika, Melanie , Nagy, Gabor , Srajer, Johannes , Oostenbrink, Chris , Chen, Xiaodong , Knoll, Wolfgang , Bo, Liedberg , Nowak, Christoph . "Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Zou, Changji , Larisika, Melanie , Nagy, Gabor , Srajer, Johannes , Oostenbrink, Chris , Chen, Xiaodong , Knoll, Wolfgang , Bo, Liedberg , Nowak, Christoph . Two-dimensional heterospectral correlation analysis of the redox-induced conformational transition in cytochrome c using surface-enhanced Raman and infrared absorption spectroscopies on a two-layer gold surface. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.