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In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon’s fibroblasts : comparisons with mitomycin C

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon’s fibroblasts : comparisons with mitomycin C
นักวิจัย : Seet, Li-Fong , Su, Roseline , Toh, Li Zhen , Wong, Tina T.
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2554
อ้างอิง : Seet, L.-F., Su, R., Toh, L. Z., & Wong, T. T. (2012). In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon’s fibroblasts: comparisons with mitomycin C. Journal of Cellular and Molecular Medicine, 16(6), 1245-1259. , 1582-1838 , http://hdl.handle.net/10220/17837 , http://dx.doi.org/10.1111/j.1582-4934.2011.01400.x
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of cellular and molecular medicine
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Failure of glaucoma filtration surgery (GFS) is commonly attributed to scarring at the surgical site. The human Tenon’s fibroblasts (HTFs) are considered the major cell type contributing to the fibrotic response. We previously showed that SPARC (secreted protein, acidic, rich in cysteine) knockout mice had improved surgical success in a murine model of GFS. To understand the mechanisms of SPARC deficiency in delaying subconjunctival fibrosis, we used the gene silencing approach to reduce SPARC expression in HTFs and examined parameters important for wound repair and fibrosis. Mitomycin C-treated HTFs were used for comparison. We demonstrate that SPARC-silenced HTFs showed normal proliferation and negligible cellular necrosis but were impaired in motility and collagen gel contraction. The expression of pro-fibrotic genes including collagen I, MMP-2, MMP-9, MMP-14, IL-8, MCP-1 and TGF-β2 were also reduced. Importantly, TGF-β2 failed to induce significant collagen I and fibronectin expressions in the SPARC-silenced HTFs. Together, these data demonstrate that SPARC knockdown in HTFs modulates fibroblast functions important for wound fibrosis and is therefore a promising strategy in the development of anti-scarring therapeutics.

บรรณานุกรม :
Seet, Li-Fong , Su, Roseline , Toh, Li Zhen , Wong, Tina T. . (2554). In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon’s fibroblasts : comparisons with mitomycin C.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Seet, Li-Fong , Su, Roseline , Toh, Li Zhen , Wong, Tina T. . 2554. "In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon’s fibroblasts : comparisons with mitomycin C".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Seet, Li-Fong , Su, Roseline , Toh, Li Zhen , Wong, Tina T. . "In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon’s fibroblasts : comparisons with mitomycin C."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2554. Print.
Seet, Li-Fong , Su, Roseline , Toh, Li Zhen , Wong, Tina T. . In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon’s fibroblasts : comparisons with mitomycin C. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2554.