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The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation
นักวิจัย : Zhang, Q. , Hunke, C. , Yau, Yin Hoe , Seow, V. , Lee, S. , Tanner, L. B. , Guan, X. L. , Wenk, M. R. , Fibriansah, G. , Chew, P. L. , Kukkaro, P. , Biuković, Goran , Shi, P.-Y. , Shochat, Susana Geifman , Grüber, Gerhard , Lok, S.-M.
คำค้น : DRNTU::Science::Biological sciences::Biochemistry
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Zhang, Q., Hunke, C., Yau, Y. H., Seow, V., Lee, S., Tanner, L. B., et al. (2012). The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation. Journal of biological chemistry, 287(48), 40525-40534. , http://hdl.handle.net/10220/17653 , http://dx.doi.org/10.1074/jbc.M112.384446
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of biological chemistry
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Newly assembled dengue viruses (DENV) undergo maturation to become infectious particles. The maturation process involves major rearrangement of virus surface premembrane (prM) and envelope (E) proteins. The prM-E complexes on immature viruses are first assembled as trimeric spikes in the neutral pH environment of the endoplasmic reticulum. When the virus is transported to the low pH environment of the exosomes, these spikes rearrange into dimeric structures, which lie parallel to the virus lipid envelope. The proteins involved in driving this process are unknown. Previous cryoelectron microscopy studies of the mature DENV showed that the prM-stem region (residues 111–131) is membrane-associated and may interact with the E proteins. Here we investigated the prM-stem region in modulating the virus maturation process. The binding of the prM-stem region to the E protein was shown to increase significantly at low pH compared with neutral pH in ELISAs and surface plasmon resonance studies. In addition, the affinity of the prM-stem region for the liposome, as measured by fluorescence correlation spectroscopy, was also increased when pH is lowered. These results suggest that the prM-stem region forms a tight association with the virus membrane and attracts the associated E protein in the low pH environment of exosomes. This will lead to the surface protein rearrangement observed during maturation.

บรรณานุกรม :
Zhang, Q. , Hunke, C. , Yau, Yin Hoe , Seow, V. , Lee, S. , Tanner, L. B. , Guan, X. L. , Wenk, M. R. , Fibriansah, G. , Chew, P. L. , Kukkaro, P. , Biuković, Goran , Shi, P.-Y. , Shochat, Susana Geifman , Grüber, Gerhard , Lok, S.-M. . (2555). The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zhang, Q. , Hunke, C. , Yau, Yin Hoe , Seow, V. , Lee, S. , Tanner, L. B. , Guan, X. L. , Wenk, M. R. , Fibriansah, G. , Chew, P. L. , Kukkaro, P. , Biuković, Goran , Shi, P.-Y. , Shochat, Susana Geifman , Grüber, Gerhard , Lok, S.-M. . 2555. "The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zhang, Q. , Hunke, C. , Yau, Yin Hoe , Seow, V. , Lee, S. , Tanner, L. B. , Guan, X. L. , Wenk, M. R. , Fibriansah, G. , Chew, P. L. , Kukkaro, P. , Biuković, Goran , Shi, P.-Y. , Shochat, Susana Geifman , Grüber, Gerhard , Lok, S.-M. . "The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Zhang, Q. , Hunke, C. , Yau, Yin Hoe , Seow, V. , Lee, S. , Tanner, L. B. , Guan, X. L. , Wenk, M. R. , Fibriansah, G. , Chew, P. L. , Kukkaro, P. , Biuković, Goran , Shi, P.-Y. , Shochat, Susana Geifman , Grüber, Gerhard , Lok, S.-M. . The stem region of premembrane protein plays an important role in the virus surface protein rearrangement during dengue maturation. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.