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Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities
นักวิจัย : Nguyen, Giang Kien Truc , Lim, Wei Han , Nguyen, Phuong Quoc Thuc , Tam, James P.
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Nguyen, G. K. T., Lim, W. H., Nguyen, P. Q. T., & Tam, J. P. (2012). Novel Cyclotides and Uncyclotides with Highly Shortened Precursors from Chassalia chartacea and Effects of Methionine Oxidation on Bioactivities. Journal of Biological Chemistry, 287(21), 17598-17607. , http://hdl.handle.net/10220/17059 , http://dx.doi.org/10.1074/jbc.M111.338970
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of biological chemistry
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Cyclotides are a new class of plant biologics that display a diverse range of bioactivities with therapeutic potentials. They possess an unusual end-to-end cyclic backbone combined with a cystine knot arrangement, making them exceptionally stable to heat, chemical and enzymatic degradation. Currently, >200 cyclotides have been discovered but only three naturally occurring linear variants (also known as uncyclotides) have been isolated. In this study, we report the discovery of 18 novel peptides, chassatides C1 to C18, composed of 14 new cyclotides and four uncyclotides from Chassalia chartacea (Rubiaceae family). Thus far, this is the largest number of uncyclotides being reported in a single species. Activity testing showed that the uncyclotides not only retain the effectiveness but also are the most potent chassatides in the assays for antimicrobial, cytotoxic, and hemolytic activities. Genetic characterization of novel chassatides revealed that they have the shortest precursors of all known cyclotides hitherto isolated, which represents a new class of cyclotide precursors. This is the first report of cyclotide genes in a second genus, the Chassalia, other than the Hedyotis (Oldenlandia) of the Rubiaceae family. In addition, we also report the characterization of two Met-oxidized derivatives of chassatides C2 and C11. The oxidation of Met residue causes loss of bioactivities, strengthening the importance of the hydrophobic patch for membrane interaction.

บรรณานุกรม :
Nguyen, Giang Kien Truc , Lim, Wei Han , Nguyen, Phuong Quoc Thuc , Tam, James P. . (2555). Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Nguyen, Giang Kien Truc , Lim, Wei Han , Nguyen, Phuong Quoc Thuc , Tam, James P. . 2555. "Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Nguyen, Giang Kien Truc , Lim, Wei Han , Nguyen, Phuong Quoc Thuc , Tam, James P. . "Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Nguyen, Giang Kien Truc , Lim, Wei Han , Nguyen, Phuong Quoc Thuc , Tam, James P. . Novel cyclotides and uncyclotides with highly shortened precursors from Chassalia chartacea and effects of methionine oxidation on bioactivities. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.